Rabbit Anti-PSME3 Polyclonal Antibody#abs135594
Please note that the price provided is for your reference only. For further information and detailed pricing, please reach out to our seller Vecent. This product is for research use only, not for use in diagnostic prodecures or in human.
Description
| Catalog-specification | Delivery time | USD price |
abs135594-100ug | 1-2 Weeks | 301.0 |
abs135594-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided is for your reference only. For further information and detailed pricing, please reach out to our seller Vecent.
| Overview | |
catalog | abs135594 |
Description | The 20S proteasome serves as the primary enzyme complex responsible for breaking down proteins within cells. To aid in its activity, three prominent protein complexes known as PA700, PA28, and PA200 act as activators for the 20S proteasome. Among these, PA28 consists of three evolutionarily conserved subunits: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β combine to form a heteroheptameric complex that facilitates binding to the opening site(s) of the 20S complex. This PA28α/β partnership is found throughout the cell and plays a crucial role in MHC class I antigen presentation by aiding in the creation of antigenic peptides derived from foreign proteins (2). On the other hand, PA28γ exists as a homoheptamer and primarily resides in the nucleus. The PA28γ complex functions by linking with specific nuclear target proteins and directing them towards the 20S proteasome for subsequent degradation (3,4). |
| Other names | The gamma subunit of the 11S regulator complex, also known as proteasome activator subunit 3 (PA28 gamma or Ki), is an important component involved in the activation of the multicatalytic protease. It is also referred to as the Ki antigen or Ki nuclear autoantigen. PA28 gamma plays a crucial role in regulating the proteasome, which is responsible for protein degradation. This subunit, denoted as PSME3 or REG gamma, is part of the proteasome activator complex and is essential for its proper functioning. Rearrangement of the given information: PA28 gamma, also known as the gamma subunit of the 11S regulator complex or proteasome activator subunit 3, is a vital component involved in multicatalytic protease activation. It is referred to as Ki antigen or Ki nuclear autoantigen. PA28 gamma plays a critical role in regulating the proteasome, responsible for protein degradation. The subunit, known as PSME3 or REG gamma, is essential for the proper functioning of the proteasome activator complex. |
| Source | Rabbit |
| Specificity | The PSME3 Antibody is designed to identify total PSME3 protein levels in cells. It specifically detects endogenous levels of PSME3. By utilizing this antibody, researchers can accurately quantify the amount of PSME3 present in their samples. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Rabbit;Sheep;Dog;Bovine;Horse; |
| Antigen | PSME3 |
| Application | ，： IHC 1:50-1:200，WB 1:500-1:2000，ELISA(peptide) 1:20000-1:40000。 |
| Immunogen | A synthesized peptide derived from human PSME3. |
| MW | 30kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978). |
| Tissue specificity | Up-regulated in thyroid carcinoma cells. |
| Posttranslational modification | Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2.Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1. |
| Celluar localization | Cytosol;Nucleus; |
| UniPort | P61289 |
| Western blot analysis of extracts of variouscell lines, using PSME3 antibody|Immunohistochemistry of paraffin-embedded Colon cancer using PSME3 Antibody. |
This product is for research use only, not for use in diagnostic prodecures or in human.
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