Rabbit Anti-FOXO1 Polyclonal Antibody #abs124255

abs124255-50ul

In Stock

201

abs124255-100ul

In Stock

301

Other names

FOXO1, also known as Forkhead box protein O1, is a crucial transcription factor that plays an essential role in regulating several cellular processes, including cell cycle regulation, apoptosis, glucose metabolism, and oxidative stress response. Afxh, AI876417, FKHR, Fkhr1, Foxo1a, Forkhead, FKH1, FKH1, FKH1, FKHR, and Forkhead box O1 are some of the alternative names for FOXO1.
FOXO1 is highly expressed in several tissues, including the liver, muscle, adipose tissue, and pancreatic β cells. Its activity is tightly regulated by post-translational modifications, including phosphorylation, acetylation, and ubiquitination, which determine its ability to interact with other transcription factors and modulate gene expression.
Recent studies have reported that dysregulation of FOXO1 can lead to several pathological conditions, including cancer, diabetes, and aging. Furthermore, studies have demonstrated the critical role of FOXO1 in stem cell self-renewal and differentiation.
In conclusion, FOXO1 is a crucial transcription factor that plays a vital role in regulating several cellular processes and is closely involved in the development of several pathological conditions. Therefore, understanding the mechanisms that regulate the activity of FOXO1 may pave the way for the development of novel therapeutic strategies for the treatment of various diseases.

Source

Rabbit

Specificity

FOXO1

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;

Application

The recommended dilutions for WB, ELISA, IHC-P, IHC-F, and IF are as follows:
- WB: 1:500-2000
- ELISA: 1:500-1000
- IHC-P: 1:400-800
- IHC-F: 1:400-800
- IF: 1:100-500
Please note that for paraffin sections, antigen retrieval is required.

Immunogen

A KLH-conjugated synthetic peptide has been developed from the human FOXO1 protein sequence. Specifically, the peptide sequence covers amino acids 201-300 out of a total of 655 amino acids present in FOXO1. This synthetic peptide is intended for use in research and immunological applications. Its design ensures high similarity to the original FOXO1 protein sequence in order to maximize its effectiveness in binding to antibodies and generating immune responses.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

-20°C，/。

Storage buffer

How about this:
To create a solution with a pH of 7.4, we combined 0.01M Tris-buffered saline with 1% bovine serum albumin, 0.03% Proclin300, and 50% glycerol. This results in a highly effective mixture that can be used in a variety of applications.

Research area

Tumor immunology transcription regulator

Target

Background

This particular gene is part of the forkhead family of transcription factors, characterized by its unique forkhead domain. Although the precise function of this gene has not yet been fully established, it may have a role in the growth and differentiation of myogenic cells. Its translocation with PAX3 has been linked to alveolar rhabdomyosarcoma, a type of cancer that affects muscles. The information provided by RefSeq suggests that more research is needed to truly understand the significance of this gene and its potential implications in disease.

Celluar localization

Nucleus cytoplasm

UniPort

Q12778