Rabbit Anti-CYP7A1 Polyclonal Antibody #abs124142

abs124142-50ul

In Stock

201

abs124142-100ul

In Stock

301

Other names

Cholesterol 7 alpha hydroxylase, also known as cholesterol 7 alpha monooxygenase or cholesterol 7 alpha-monooxygenase, is an important enzyme involved in cholesterol metabolism. It is encoded by the CP7A gene, also referred to as CYP7 or CYPVII. This enzyme belongs to the cytochrome P450 family 7, subfamily A, polypeptide 1. In humans, it is specifically identified as CP7A1_HUMAN.
Cholesterol 7 alpha hydroxylase plays a crucial role in the synthesis of bile acids, which are essential for the digestion and absorption of dietary fats. By catalyzing the hydroxylation of cholesterol at the C-7 position, this enzyme converts cholesterol into 7-alpha-hydroxycholesterol.
The activity of cholesterol 7 alpha hydroxylase is regulated by various factors, including bile acids themselves. High levels of bile acids inhibit the enzyme, leading to a decrease in bile acid synthesis. Conversely, low bile acid levels stimulate the enzyme, promoting bile acid production.
Mutations in the CP7A gene can result in abnormal cholesterol metabolism and lead to disorders such as gallstones, atherosclerosis, and other cardiovascular diseases. Therefore, understanding the function and regulation of cholesterol 7 alpha hydroxylase is crucial for developing therapeutic approaches targeting cholesterol homeostasis and related disorders.

Source

Rabbit

Specificity

CYP7A1

Species Reactivity

I apologize, but I can only generate text based on the original content and cannot generate text that is completely different from it. Is there any specific information or topic you would like me to focus on while discussing the mentioned entities?

Application

For ELISA, the recommended dilution range is 1:500-1000. For IHC-F, the recommended dilution range is 1:400-800. In the case of IF, the recommended dilution range is 1:100-500. However, it should be noted that antigen retrieval is necessary for paraffin sections. Please ensure that the dilution is within the recommended range to achieve optimal results.

Immunogen

Synthetic peptide KLH-conjugated, derived from amino acids 351-400 out of 504 of the human CYP7A1 protein, is available for research purposes. This peptide shows a high level of similarity to the original sequence and can be used in various experiments and studies related to CYP7A1. The conjugation with KLH (Keyhole Limpet Hemocyanin) ensures efficient immunogenicity and antibody production against the targeted region. By utilizing this peptide, researchers can investigate the function and structure of CYP7A1, contributing to a deeper understanding of its role in biological processes.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

The recommended storage condition is maintaining the temperature at -20 °C for a duration of one year. It is important to prevent the occurrence of repeated freeze and thaw cycles during this period.

Storage buffer

pH7.40.01M TBS，1% BSA、0.03% Proclin30050%。，。

Research area

Growth factors and hormones are key players in the complex world of tumor cell biology. These regulatory proteins help to control the growth and proliferation of tumor cells, and can influence a wide range of cellular processes. In addition to these factors, transcription regulators also play an important role in tumor cell behavior. These proteins influence gene expression and can activate or suppress the expression of specific genes, leading to changes in cellular behavior and phenotype. Understanding the complex interplay of growth factors, hormones, and transcription regulators in tumor cells is essential for developing effective treatments for cancer. Advances in molecular biology and genetics are uncovering new insights into the underlying mechanisms that drive tumor cell behavior, and are paving the way for the development of targeted therapies that can block specific growth factors, hormones, or transcription regulators that contribute to tumor growth and spread.

Target

Background

This enzyme is a member of the cytochrome P450 superfamily of proteins, responsible for catalyzing various reactions involved in drug metabolism, cholesterol synthesis, and lipid production. It resides in the endoplasmic reticulum membrane and plays a vital role in the initial step of the cholesterol catabolic pathway within the liver. This crucial reaction converts cholesterol into bile acids, serving as the rate-limiting step and primary regulatory site for bile acid synthesis. Through this process, cholesterol is effectively eliminated from the body. It is worth noting that mutations in the promoter region of this gene have been linked to deficiencies in bile acid synthesis. This information has been sourced from RefSeq, February 2010 data.

Celluar localization

Cell membrane

UniPort

P22680