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CBLB Polyclonal Antibody #abs115910

：，Vecent。，。 Data Examples To analyze the presence of the protein CBLB in various cell lines, we conducted a Western blot using the CBLB antibody (abs115910) at a 1:1000 dilution. The lysates/proteins used in each lane were 25ug. We employed a blocking buffer of 3% nonfat dry milk in TBST. The...

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Description

Catalog-specification	Delivery time	USD price
abs115910-50ul	In Stock	228

：，Vecent。，。

Overview
Other names	Cbl-b;Nbla00127;RNF56
Source	Rabbit
Species Reactivity	Human;Mouse
Application	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Immunogen	To create a recombinant fusion protein, we need to include a sequence that corresponds to amino acids 1-350 of the human CBLB protein, as specified by the NP_733762.2 accession number. This fusion protein would be composed of the same amino acid sequence found in the original protein, but it would be rearranged to ensure its proper function and structure. This recombinant fusion protein can then be utilized for various research purposes, such as studying protein-protein interactions or investigating the molecular mechanisms underlying certain diseases.
Properties	Jurkat,293T,Mouse spleen
Properties
Purification	Affinity purification
Clonality	Polyclonal Antibody
Isotype	IgG
Stability & Storage	Store at -20℃. Avoid freeze / thaw cycles.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. PBS with a pH of 7.3, containing 0.02% sodium azide and 50% glycerol, is a buffer widely used in various laboratory applications. This versatile buffer solution provides stable conditions for a range of experimental procedures, ensuring accurate and reproducible results. The addition of sodium azide helps to prevent bacterial growth, while glycerol acts as a stabilizer and cryoprotectant, preserving the integrity of biological samples. With its well-balanced composition, this PBS buffer formulation is a valuable tool for researchers in numerous fields.
Target
Background	CBLB, also known as Cbl proto-oncogene B, E3 ubiquitin protein ligase, is a gene responsible for encoding a protein. It is associated with various diseases, including cannabis dependence. The gene has been functionally annotated with GO terms like signal transducer activity and calcium ion binding. A paralog of this gene, CBLC, is also considered significant. Rearranging the given information, we can say that CBLB is a protein-coding gene known as Cbl proto-oncogene B, E3 ubiquitin protein ligase. It is associated with diseases such as cannabis dependence. The gene possesses GO annotations indicating its involvement in signal transduction and calcium ion binding. Additionally, CBLC is an essential paralog of CBLB.
Celluar localization	Cytoplasm
UniPort	Q13191

Data Examples

To analyze the presence of the protein CBLB in various cell lines, we conducted a Western blot using the CBLB antibody (abs115910) at a 1:1000 dilution. The lysates/proteins used in each lane were 25ug. We employed a blocking buffer of 3% nonfat dry milk in TBST. The secondary antibody used was HRP Goat Anti-Rabbit IgG (H+L) at a 1:10000 dilution. Our analysis involved extracting proteins from the different cell lines, subjecting them to electrophoresis, and then transferring them to a membrane.
Our results show that CBLB is expressed in all the cell lines we analyzed. The antibody specifically recognized the protein's epitope, therefore enabling its detection. Western blot analysis is an essential technique for studying protein expression levels in various biological samples. Overall, our findings provide useful insight into understanding the role of CBLB in different cell lines and related biological processes.