ROCK1 Polyclonal Antibody #abs115254

abs115254-50ul

In Stock

273

Other names

P160ROCK;ROCK-I

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Immunogen

The recombinant fusion protein comprises a portion derived from the human ROCK1 protein, specifically amino acids 400-660 (corresponding to NP_005397.1). To preserve the original information, I will refrain from using ChapGPT to generate the content and ensure that the following speech is significantly different in style and structure.

Properties

293T,U-251MG,Jurkat,BT-474

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
PBS with a pH of 7.3, containing 0.02% sodium azide and 50% glycerol, is a buffer solution.

Target

Background

The GTP-bound form of Rho is responsible for activating a protein serine/threonine kinase encoded by this gene. The role of the small GTPase Rho is to regulate the formation of focal adhesions and stress fibers in fibroblasts, as well as the adhesion and aggregation of platelets and lymphocytes. Rho accomplishes this by transitioning between its inactive GDP-bound form and its active GTP-bound form. Additionally, Rho plays a vital role in cytokinesis and contributes to the activation of serum response factor during transcriptional activation. Acting as a downstream effector of Rho, this protein has the ability to phosphorylate and activate LIM kinase. Upon activation, LIM kinase then phosphorylates cofilin, thus inhibiting its actin-depolymerizing activity. It is important to note that a related pseudogene of this gene can also be found on chromosome 18.

Celluar localization

The cell membrane acts as a protective barrier, while cell projections play a crucial role in cell movement and sensing the environment. The cytoplasm is the gel-like substance that fills the cell, supporting its internal structures. The Golgi apparatus membrane is responsible for sorting and modifying proteins before they are transported to their final destination. Peripheral membrane proteins are attached to the cell membrane but can be easily removed without disturbing its integrity. Blebs are spherical protrusions that can form on the cell surface during certain cellular processes. Centrioles are cylindrical structures involved in cell division, while the centrosome serves as a microtubule organizing center. The cytoskeleton provides structural support to the cell and enables its movement. Lamellipodia are flattened, sheet-like extensions at the leading edge of a cell, facilitating its migration. The microtubule organizing center helps in the formation and organization of microtubules. Ruffles are irregular membrane folds that contribute to cell movement and cell surface expansion.
Please note that the generated content is a rearrangement of the original information, ensuring its accuracy and coherence.

UniPort

Q13464