Recombinant Mouse CD28//TP44 (C-Fc-6His) #abs04037

abs04037-10ug

1-2 Weeks

46

abs04037-50ug

1-2 Weeks

123

abs04037-500ug

1-2 Weeks

619

abs04037-1mg

1-2 Weeks

801

Description

Our Mammalian expression system is utilized to produce Recombinant Mouse CD28, wherein the target gene that codes for Asn20-Lys149 is expressed with a Fc, 6His tag positioned at the C-terminus.

Other names

T-cell-specific surface glycoprotein CD28，CD28

Source

Human Cells

Format

The solution of 20mM PB, 150mM NaCl, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to obtain a dried form.

Properties

aa_sequence

LSGNHFHFHQDKDVYENYLVKEPADPQPLHVNLAFTERTQSSKIVEMLMHDVDRSINLVHNCGESFNOETLKEDVCTSGYEDDFNFITALKQSR VPMPRQHEGSCWYPILKNSYYVFDEENVGVKVTGVLTVKWCNDTASEFQFEVTEYLRRINYTLRSHKYNKSGGPIASGGPSLDGYVKLQWMK WNTVNSHTVYDNWFDYNERFSEPCGANLDFQSCSTYMVECEFTKPQHRONNLKGSTVQIDVATFVVNPLSCGFEPLHTDPKEISGKM TVVTSHPPGPWGNQSFFNKWEYNEKDNPYKITVSKAfLKPTQFEQEYVVHLCTKCLEDLSCVDSPNNPLRQNKVRDQYQFPVSGMTPA DYYPWPLGEQNKSCPKIKRVLSHSIDFYWWQNFVITDKPWVDLGTQNKINLTYGFEAAPPLAKMTLDEHFSYURLFIKCQNEVVMRFGCCES IVFNSVGPSLNRRPEHYEKEVSQGKKGATNDISKSKCQF Ney-NNEQSPLLVVDSNEVSLSCRYSYNLLAKEFRASLYKGVNSDVEVCVGNGNFTYQPQFRSN AEFNCDGDFDNETVTFRLWNLHVNHTDIYFCKIEFMYPPPYLDNERSNGTIIHIKEKHLCH TQSSPKVDDIEGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVS HEDEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTIS KAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDI EVESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS S

Concentration

SDS-PAGE，95%。，，。

Endotoxin_level

The LAL test confirms that the amount of endotoxin present is less than 0.1 ng per microgram or 1 International Unit per microgram.

Reconstitution

Make sure to always centrifuge the tubes before opening to ensure optimal results. Avoid using the vortex or pipetting methods for mixing. It is highly advised not to reconstitute the protein to a concentration lower than 100 μg/ml. To dissolve the lyophilized protein, use ddH2O. Additionally, it is important to aliquot the reconstituted solution to minimize freeze-thaw cycles.

Stability & Storage

For optimal storage of lyophilized protein, it is recommended to keep it at a temperature below -20°C. However, if necessary, the protein can be stored at room temperature for up to 3 weeks without losing its stability.
When the protein is reconstituted, it should be stored at a temperature of 4-7°C for 2-7 days to ensure its longevity. If the sample needs to be stored for a longer period, then it should be aliquoted and kept at a temperature below -20°C for up to 3 months.
By taking these precautions, one can ensure the protein's stability and prevent any degradation that may occur due to incorrect storage conditions. Thus, it is vital to follow these guidelines to preserve the protein's integrity and ensure its usefulness in various applications.

Target

Background

T-cell-specific surface glycoprotein CD28, contains an Ig-like V-type domain. CD28 is one of the proteins expressed on T cells that provide co-stimulatory signals, which are required for their activation. It is the receptor for CD80 and CD86. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. It involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Accession

P31041