Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

CD9 Antibody (Center) #abs102483

Description

Catalog-specification	Delivery time	USD price
abs102483-50ul	In Stock	228

Please note that the price provided is only for your reference. For more detailed pricing information, please contact our seller, Vecent. It is important to clarify that any similar content generated by rearranging the original text is based on the same information. However, please refrain from using ChapGPT to generate identical content, and instead, utilize the language model to produce completely unique, original content.

Overview
Description	Purified Rabbit Polyclonal Antibody (Pab)
Other names	The CD9 antigen, also known as the 5H9 antigen, has been identified as a cell growth-inhibiting gene 2 protein. It is also referred to as the leukocyte antigen MIC3, motility-related protein MRP-1, tetraspanin-29, Tspan-29, and p24. CD9 plays a crucial role in facilitating cell adhesion, migration, and signaling processes, and it participates in the formation of various cellular structures. MIC3 is predominantly expressed in immune cells and helps in modulating their functions. TSPAN29 refers to the tetraspanin family member that is closely related to CD9, and it is involved in various cellular processes like cell migration and proliferation. CD9, MIC3, and TSPAN29 are potential biomarkers and therapeutic targets for several diseases like cancer, cardiovascular disorders, and autoimmune diseases.
Source	Rabbit
Specificity	The CD9 antibody has been produced from rabbits that were immunized using a synthetic peptide which has been conjugated with KLH. This peptide corresponds to the 115-145 amino acids located in the central region of human CD9. To ensure the generated content is highly similar, I have rearranged the given information while still retaining the original text meaning.
Species Reactivity	Human
Application	IF~~1:25 WB~~1:2000
Properties
Clonality	Polyclonal Antibody
Isotype	Rabbit Ig
Stability & Storage	To preserve its quality, it is recommended to keep this item refrigerated at 2-8°C and not exceed a storage period of two weeks. For extended periods of storage, it is advisable to store it in small aliquots at a temperature of -20°C to prevent freeze-thaw cycles. This ensures that the specimen remains in good condition and its properties are not compromised.
Storage buffer	This particular antibody is derived from a polyclonal source and has undergone a purification process to remove impurities. It is supplied in a solution of PBS (phosphate-buffered saline) with the addition of 0.09% (W/V) sodium azide. To obtain the purified antibody, a method known as Saturated Ammonium Sulfate (SAS) precipitation was employed, followed by dialysis against PBS to remove excess salts and other contaminants.
Research area	Developmental biology is the study of how organisms grow and develop from a single cell into a fully formed organism. A key aspect of this field is understanding the various signaling pathways that control cellular development and tissue formation. Cardiovascular function is critical for maintaining overall health and wellness, as the heart and blood vessels are responsible for transporting essential nutrients and oxygen throughout the body. One important area of study in this field is investigating the various factors that influence cardiovascular disease risk. Immunology is the study of the body's immune system and how it functions to protect against harmful pathogens and other foreign substances. This discipline is particularly relevant for understanding how vaccines work and developing new treatments for infectious diseases and autoimmune disorders. Signal transduction refers to the process by which cells communicate with one another through chemical signals and molecular pathways. The study of signal transduction is crucial for understanding how cells respond to their environment, and how this communication contributes to disease processes and other physiological phenomena.
Target
Background	Platelet activation and aggregation are controlled by this protein that also plays a crucial role in the formation of paranodal junctions. It is involved in various cellular processes including cell adhesion, cell motility, and tumor metastasis. Moreover, it is essential for sperm-egg fusion.
Celluar localization	A multi-pass membrane protein can be found within a membrane, such as the cell membrane. These proteins traverse back and forth through the membrane multiple times, allowing them to perform important functions within the cell. The structure of multi-pass membrane proteins can be complex, and the number of passes through the membrane can vary. However, these proteins play a crucial role in maintaining the integrity and function of the cell membrane.
UniPort	P21926
References
References	Ovalle,S., Int. J. Cancer 121 (10), 2140-2152 (2007) Kovalenko,O.V., Mol. Cell Proteomics 6 (11), 1855-1867 (2007) Abache,T., J. Cell. Biochem. 102 (3), 650-664 (2007) Horejsi,V., FEBS Lett. 288 (1-2), 1-4 (1991)

Data Examples

Anti-CD9 Antibody (Center)at 1:2000 dilution + MCF-7 whole cell lysatesLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center)(Cat. #abs102483). abs102483 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Western blot analysis of lysate from mouse kidney tissue lysate, using CD9 Antibody (Center)(Cat. #abs102483). abs102483 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using CD9 Antibody (Center) (Cat. #abs102483). abs102483 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot analysis of lysate from Hela cell line, using CD9 Antibody (Center)(Cat. #abs102483). abs102483 was diluted at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.