HMGCR Polyclonal Antibody #abs115668

abs115668-50ul

In Stock

228

Other names

LDLCQ3

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:1000 - 1:4000IHC 1:50 - 1:100

Immunogen

One possible arrangement of the content could be:
A fusion protein has been created with a section that matches amino acids 629-888 from the human HMGCR protein (NP_000850.1). This recombinant protein combines different molecular components to create a new biochemical entity with specific properties and functions. The selected HMGCR fragment is known to be important for cholesterol biosynthesis and is often targeted by drugs that lower cholesterol levels. By using recombinant DNA technology, scientists can produce large amounts of this fusion protein for research or therapeutic purposes. The precise design and production of the fusion protein can influence its stability, activity, and specificity, as well as its potential interactions with other molecules in a cellular context. Understanding the structure and behavior of recombinant fusion proteins is an ongoing area of biotechnology research.

Properties

HepG2,HeLa,SH-SY5Y,BT-474,Mouse spleen,Rat liver

Properties

Purification

Affinity purification

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
A solution known as buffer PBS containing 0.02% sodium azide and 50% glycerol, with a pH level of 7.3, is commonly utilized in laboratory settings. This composition is frequently used in various applications and is an essential component of many laboratory experiments. The inclusion of sodium azide helps to prevent bacterial growth, while the addition of glycerol offers protection to samples from harsh environmental conditions. The overall pH level of 7.3 is optimized to ensure optimal functionality in a range of biological and chemical reactions. In summary, buffer PBS is a widely employed solution in laboratory research due to its versatility and crucial role in generating accurate experimental results.

Target

Background

The rate-limiting enzyme for cholesterol synthesis in mammalian cells is HMGCR. It is regulated through a negative feedback mechanism involving sterols and non-sterol metabolites derived from mevalonate, which is the product of the reaction catalyzed by reductase. The suppression of HMGCR is normally achieved by cholesterol acquired from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. By inducing the expression of LDL receptors in the liver, competitive inhibitors of the reductase can increase the catabolism of plasma LDL, leading to lower levels of cholesterol in the bloodstream. This decrease in cholesterol concentration is a crucial factor in reducing the risk of atherosclerosis, a cardiovascular disease. It is worth noting that there are different isoforms of the HMGCR gene, which are encoded by alternatively spliced transcript variants.

Celluar localization

A multi-pass membrane protein can be found embedded within the endoplasmic reticulum membrane. To create similar content, we can rearrange the information from the original text to read: The endoplasmic reticulum membrane contains a multi-pass membrane protein, which is integrated within the membranes.

UniPort

P04035