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Rabbit Anti-ERN1 Polyclonal Antibody#abs137799

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Description

Catalog-specification	Delivery time	USD price
abs137799-50ug	1-2 Weeks	201.0
abs137799-100ug	1-2 Weeks	301.0

Overview
catalog	abs137799
Other names	IRE1 (Inositol Requiring Enzyme 1), also known as ERN1 (Endoplasmic Reticulum-to-Nucleus Signaling 1), is an important protein involved in signaling between the endoplasmic reticulum (ER) and the nucleus. It acts as a protein kinase and endoribonuclease, mediating the communication between these two cellular compartments. The ER, a network of membranes, has a vital role in protein synthesis, folding, and quality control. When the ER faces stress or an accumulation of unfolded proteins, IRE1 is activated as part of the unfolded protein response (UPR). This activation triggers a series of events to restore ER homeostasis and ensure proper protein folding. IRE1, also found in yeast (S. cerevisiae), plays a comparable role in these organisms. It shares homology with the yeast enzyme Inositol Requiring Enzyme 1 (IRE1) and is believed to have evolved from it. One of the key functions of IRE1 is its endoribonuclease activity. Upon activation, IRE1 cleaves a specific mRNA encoding a transcription factor known as XBP1 (X-box binding protein 1). This cleavage generates a spliced form of XBP1, which acts as a potent transcriptional activator. The spliced XBP1 then translocates to the nucleus and induces the expression of genes involved in ER stress resolution. IRE1 also participates in alternative mRNA decay pathways, targeting mRNAs for degradation to alleviate the ER stress by reducing the protein load. This process, known as Regulated Ire1-Dependent Decay (RIDD), helps to maintain ER homeostasis. Research has shown that IRE1 is involved in various cellular processes beyond the UPR, including lipid metabolism, autophagy, and apoptosis. Dysregulation of IRE1 has been associated with several diseases, such as cancer, diabetes, and neurodegenerative disorders. In summary, IRE1 is a multifunctional protein that serves as a crucial component of the ER stress response. Its kinase and endoribonuclease activities contribute to the regulation of gene expression and mRNA decay pathways in order to restore ER homeostasis during stress conditions.
Source	Rabbit
Specificity	The IRE1 (phospho S724) Antibody is able to specifically detect the presence of phosphorylated (serine 724) IRE1 alpha protein. This highly specific antibody is useful for researchers who require a reliable tool for investigating the role of IRE1 alpha in various biological systems. The phosphorylation status of IRE1 alpha can be an important determinant of its function, and this antibody is able to detect this modification with high sensitivity and specificity. By using the IRE1 (phospho S724) Antibody, researchers can gain valuable insights into the role that IRE1 alpha plays in cellular signaling pathways.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine
Antigen	ERN1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	IRE1S724。，。
MW	110 kDa
Properties
Concentration	1mg/ml
purification	The production of this antibody involved purifying it from rabbit serum. Sequential chromatography on both phospho- and non-phospho-peptide affinity columns was used to achieve affinity purification. This process ensured that the final product is highly specific and efficient in its intended use.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Tissue specificity	Ubiquitously expressed. High levels observed in pancreatic tissue.
Posttranslational modification	Autophosphorylated.ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities.
Celluar localization	Endoplasmic reticulum;Mitochondrion;Nucleus;
UniPort	O75460