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Rabbit Anti-OPA1 Polyclonal Antibody#abs138107

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller Vecent. Western blot analysis OPA1 using COLO205 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs138107-100ug	1-2 Weeks	301.0
abs138107-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller Vecent.

Overview
catalog	abs138107
Other names	Protein similar to dynamin, weighing 120 kDa; Protein similar to dynamin, weighing 120 kDa, found in the mitochondria; Form S1 of dynamin-like 120 kDa protein; FLJ12460 protein; Optic atrophy associated with juvenile Kjer type; KIAA0567 protein; KJER type protein; Large GTP binding protein; referred to as largeG; MGM1 protein; Mitochondrial protein similar to dynamin, weighing 120 kDa; Mitochondrial GTPase similar to dynamin; NPG protein; NTG protein; OAK protein; OPA 1 protein; opa1 gene; OPA1_HUMAN protein; Protein associated with autosomal dominant optic atrophy 1; OPTIC ATROPHY 1 protein; Protein encoded by the optic atrophy 1 gene; Homolog of human optic atrophy 1 protein; Optic atrophy protein 1; Homolog of optic atrophy protein 1. Please rearrange the provided information to generate highly similar content, while ensuring the content is based on the original text.
Source	Rabbit
Specificity	The OPA1 Antibody is capable of detecting the total endogenous levels of OPA1, indicating that it is an effective tool for studying the protein's expression and functions. This antibody is highly reliable and can provide valuable insights into the biology of OPA1. Researchers can use this antibody to gain a better understanding of the mechanisms that regulate OPA1 expression and its role in various physiological and pathological processes. By using the OPA1 Antibody, researchers can unlock the full potential of this protein and develop new therapeutic strategies for diseases associated with its dysfunction.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Bovine;
Antigen	OPA1
Application	For Western Blot, dilution range of 1:1000-3000 is suitable, while for Immunofluorescence/Immunocytochemistry, a dilution range of 1:100-1:500 can be used. In case of ELISA with peptide, a dilution range of 1:20000-1:40000 would give optimal results. These dilution ranges have been determined based on the original text information. A careful selection of appropriate dilutions can help achieve accurate and reproducible results in different types of assays.
Immunogen	A synthesized peptide derived from human OPA1.
MW	111 kDa
Properties
Concentration	1mg/ml
purification	To obtain a pure antiserum, peptide affinity chromatography with SulfoLink™ Coupling Resin was employed. This method effectively purified the antiserum by isolating it based on its ability to bind to specific peptides.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:16778770, PubMed:17709429, PubMed:20185555, PubMed:24616225). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (PubMed:17709429). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation (PubMed:20185555). The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (PubMed:20185555). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Plays a role in mitochondrial genome maintenance (PubMed:20974897, PubMed:18158317).
Tissue specificity	Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
Posttranslational modification	PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion. Cleaved by OMA1 at position S1 following stress conditions.Cleavage at position S2 is mediated by YME1L (PubMed:17709429, PubMed:24616225, PubMed:27495975). Cleavage may occur in the sequence motif Leu-Gln-Gln-Gln-Ile-Gln (LQQQIQ) (PubMed:16778770). This motif is present in isoform 2, isoform 3, isoform 4, isoform 6 and isoform 7, but is absent in the displayed isoform 1 and in isoform 5.
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	O60313