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Rabbit Anti-LRP1 Polyclonal Antibody#abs133272

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to contact our seller, Vecent. Western blot analysis LRP1 using HepG2 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or...

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Description

Catalog-specification	Delivery time	USD price
abs133272-100ug	1-2 Weeks	301.0
abs133272-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to contact our seller, Vecent.

Overview
catalog	abs133272
Other names	LRP1, also known as A2MR or alpha 2 macroglobulin receptor, is a protein involved in various cellular processes. It is also referred to as alpha-2-macroglobulin receptor or Apolipoprotein E receptor. LRP1 acts as a receptor for multiple ligands, including CD91 antigen and IGFBP3R. LRP1 plays a significant role in lipid metabolism and clearance of low-density lipoproteins (LDL) from the circulation. It is closely related to the LDL receptor and is responsible for the internalization and degradation of LDL particles. LRP1 is also involved in the regulation of growth factor signaling, such as the TGF-beta pathway, as indicated by its alternative name, TGFBR5. The LRP1 protein consists of multiple domains and subunits, including LRP 515 and LRP 85. The intracellular domain of LRP1, known as LRP ICD, is crucial for its signaling and trafficking functions. LRP1A and MGC88725 are alternative names for LRP1. In summary, LRP1, also known as A2MR or alpha 2 macroglobulin receptor, is a versatile protein involved in lipid metabolism, growth factor signaling, and clearance of LDL particles. It mediates various cellular processes and is essential for maintaining cellular homeostasis.
Source	Rabbit
Specificity	The LRP1 Antibody is capable of detecting the entirety of LRP1 present within an organism. This detection is specific to endogenous levels, meaning it identifies natural levels of LRP1 without external manipulation.
Species Reactivity	Human;Mouse;Rat
Antigen	LRP1
Application	For Western blotting, dilutions of 1:1000-3000 should be used, while an IHC dilution of 1:200 is recommended. For ICC/IF, a dilution of 1:200 is appropriate, and for ELISA using peptide, dilutions of 1:20000-1:40000 are recommended. It is important to ensure that the dilutions used are appropriate and within the recommended range in order to obtain accurate and reproducible results. Care should also be taken to follow proper experimental protocols and controls to ensure that the results obtained are reliable and meaningful.
Immunogen	A synthesized peptide derived from human LRP1.
MW	85kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To obtain a highly refined product, the resin was employed to isolate the desired peptides from impurities.
Clonality	Polyclonal Antibody
Stability & Storage	One year of storage is recommended at -20 °C to maintain the quality of the product. It is important to avoid subjecting it to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission (PubMed:11907044, PubMed:12888553, PubMed:12713657). Acts as an alpha-2-macroglobulin receptor (PubMed:26142438).
Tissue specificity	Most abundant in liver, brain and lung.
Posttranslational modification	Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.The N-terminus is blocked.Phosphorylated on serine and threonine residues.Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.
Celluar localization	Cytosol;Endosome;Lysosome;Nucleus;Plasma Membrane;
UniPort	Q07954