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Rabbit Anti-ENO1 Polyclonal Antibody#abs133259

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Description

Catalog-specification	Delivery time	USD price
abs133259-50ug	1-2 Weeks	201.0
abs133259-100ug	1-2 Weeks	301.0

Overview
catalog	abs133259
Other names	Enolase 1, also known as 2-phospho-D-glycerate hydro-lyase or phosphopyruvate hydratase, is a key enzyme involved in glycolysis and gluconeogenesis. It is encoded by the ENO1 gene and is found in most tissues and cell types in the body. Enolase 1 is also known to be a plasminogen-binding protein, and it has been implicated in a number of biological processes, including cell migration, cell growth, and cancer progression. Enolase 1 is a multifunctional protein that exhibits various biological properties. It is involved in the regulation of gene expression, and it is known to interact with the C-myc promoter, thereby regulating the expression of the oncogene. Enolase 1 has also been shown to bind to tau-crystallin, which is implicated in cataract formation. In addition to its enzymatic functions, enolase 1 has been reported to have a role in the immune response, and it may participate in various signaling pathways by interacting with other proteins. Enolase 1 is expressed in both neural and non-neural tissues, and it has been associated with a number of human diseases. For example, enolase 1 has been implicated in the development of autoimmune diseases, as well as in the progression of some cancers. Research into the functions of enolase 1 continues to uncover the complex roles that this protein plays in the body, and these findings may lead to new therapeutic targets for a variety of diseases.
Source	Rabbit
Specificity	The ENO1 Antibody is specifically designed to identify total ENO1 levels present in the body. In essence, it helps to detect the amount of ENO1 that is naturally produced in the system. To reiterate, the antibody does not identify any other protein apart from ENO1.
Species Reactivity	Human;Mouse;Rat
Antigen	ENO1
Application	The recommended dilutions for WB range from 1:1000 to 3000, while for ICC/IF they are 1:100-500. For ELISA using a peptide, the suggested dilution is between 1:20000 to 1:40000. This dilution range is also applicable for other ELISA assays that use peptides as the antigen. These dilutions have been optimized for optimal performance and should be used as a guideline for optimal results.
Immunogen	A synthesized peptide derived from human ENO1.
MW	47kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was achieved through peptide affinity chromatography. To obtain a highly pure form of the serum, specific peptides were targeted and bound to the resin, effectively separating them from other undesirable components. This process helped to ensure that the resulting antiserum was of high quality and could be used effectively for intended experimental purposes.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20°C for up to one year. To avoid any degradation of the product, it is essential to refrain from subjecting it to repeated cycles of freezing and thawing. Take care to follow the recommended storage and handling procedures to ensure that the product remains viable and effective for its intended use.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Tissue specificity	The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Posttranslational modification	ISGylated.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P06733