Phospho-p70 S6 Kinase (Thr229) Rabbit Polyclonal Antibody#abs130853

abs130853-50ug

1-2 Weeks

201

abs130853-100ug

1-2 Weeks

301

Description

The RSK (ribosomal S6 kinase) family of serine/threonine kinases includes this particular gene as a member. This gene features two kinase catalytic domains, with each domain unrelated to the other, and it is responsible for phosphorylating multiple residues of the S6 ribosomal protein.

Other names

1) The human protein known as 70 kDa ribosomal protein S6 kinase 1, also referred to as KS6B1_HUMAN, plays a crucial role in cellular processes. It is alternatively named p70 alpha, P70 beta 1, p70 ribosomal S6 kinase alpha, p70 ribosomal S6 kinase beta 1, p70 S6 kinase alpha, P70 S6 Kinase, p70 S6 kinase alpha 1, p70 S6 kinase alpha 2, p70 S6K, p70 S6K-alpha, p70 S6KA, p70(S6K) alpha, p70(S6K)-alpha, p70-alpha, p70-S6K 1, p70-S6K, P70S6K, P70S6K1, p70S6Kb, PS6K, Ribosomal protein S6 kinase 70kDa polypeptide 1, Ribosomal protein S6 kinase beta 1, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K-beta-1, S6K1, Serine/threonine kinase 14 alpha, Serine/threonine-protein kinase 14A, and STK14A.
2) This highly versatile protein interacts with various cellular components and participates in multiple signaling pathways. Its functions include the regulation of cell growth, proliferation, survival, metabolism, and protein synthesis.
3) By rearranging and reshuffling the information from the original text, a new description can be generated:
- Ribosomal protein S6 kinase 70kDa polypeptide 1 (RPS6KB1), also known as p70 S6 Kinase, demonstrates its indispensability in fundamental cellular processes. This serine/threonine kinase, alternatively designated as KS6B1_HUMAN or S6K1, plays a pivotal role in orchestrating cell growth, proliferation, survival, and regulation of protein synthesis.
4) This altered content maintains the essence of the original text while presenting the information in a distinct and novel manner. Please note that the newly generated content is based on the provided text information but may not be an exact rearrangement of the original text.

Source

Rabbit

Specificity

The Phospho-p70 S6 Kinase (Thr229) Antibody is capable of detecting the presence of p70 S6 Kinase only if it has been phosphorylated at Threonine 229. This antibody has been developed to specifically target and identify the endogenous levels of p70 S6 Kinase that have undergone this specific type of modification, allowing for accurate and reliable results in a variety of research and diagnostic applications. By using this antibody, researchers can gain valuable insights into the complex signaling pathways and cellular processes that are regulated by this important protein kinase.

Predictive reaction species

Chicken;Rabbit;Xenopus;Dog;Horse;Sheep;Bovine

Application

The dilution ranges for WB (western blot) are 1:500-1:2000, for IHC (immunohistochemistry) are 1:50-1:200, for IF/ICC (immunofluorescence/immunocytochemistry) are 1:100-1:500, and for ELISA (enzyme-linked immunosorbent assay) using peptides are 1:20000-1:40000. These dilution ranges refer to the recommended ratios of primary antibodies to be used in each respective assay. It is important to follow these guidelines to ensure optimal detection and accurate results in experimental studies.

A man-made peptide has been developed using human p70 S6 Kinase as a basis, specifically targeting the phosphorylation site of Threonine 229. The resulting peptide is a close replica of the original and has potential for use in various applications.

Properties

1mg/ml

Purification

The highly purified antibody was obtained from rabbit serum via affinity purification using sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Clonality

Polyclonal Antibody

To maintain the quality and potency of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting it to repeated cycles of freezing and thawing, as this can affect the efficacy of the product. Therefore, it is advisable to take extra care when handling the product and store it in a stable environment to ensure its integrity and maximum longevity.

Storage buffer

Rabbit IgG is preserved in a solution containing phosphate buffered saline at pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the solution at a temperature of -20 °C. The stability of the product is guaranteed for a period of 12 months starting from the date of receipt.

Target

Background

 Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.

Tissue specificity

Widely expressed.

Celluar localization

Cytosol;Mitochondrion;Nucleus;

P23443