Rabbit Anti-ABCG8 Polyclonal Antibody #abs120422

abs120422-100ul

In Stock

301

Other names

Sterolin 2, also known as ATP binding cassette sub family G member 8 (ABCG8), sterolin 2, or MGC142217, belongs to the ATP binding cassette sub family G (WHITE) member 8. It is a vital protein involved in cellular processes related to cholesterol transport and metabolism. ABCG8 plays a crucial role in maintaining cholesterol homeostasis in the body. Its function is regulated by ATP binding and hydrolysis.
ABCG8, alternatively known as sterolin 2 or MGC142217, is an essential component of the ABCG transporters family. It is responsible for the efflux of sterols, mainly cholesterol, from hepatocytes into bile. This process prevents excessive accumulation of cholesterol in the liver and promotes its elimination through the bile. ABCG8 also collaborates with ABCG5 to form a heterodimeric transporter complex, which enhances the efflux of sterols.
The ABCG8 protein, encoded by the ABCG8 gene, is widely expressed in various tissues, including the liver, intestine, and gallbladder. It is localized in the canalicular membrane of hepatocytes and the brush border membrane of enterocytes. This subcellular localization enables ABCG8 to effectively transport sterols across these membranes.
Studies have indicated that mutations in the ABCG8 gene can lead to genetic disorders known as sitosterolemia and phytosterolemia. These conditions are characterized by impaired sterol transport and increased levels of plant sterols in the body. Consequently, affected individuals may display symptoms such as xanthomas, premature atherosclerosis, and increased risk of cardiovascular disease.
In summary, ABCG8 is an important protein involved in cholesterol metabolism and transport. Its role in maintaining cholesterol homeostasis highlights its significance in overall human health. Further research on ABCG8 and its regulation may provide insights into potential therapeutic targets for managing cholesterol-related disorders.

Source

Rabbit

Specificity

ABCG8

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;Horse;Rabbit;

Application

The recommended dilutions for WB, ELISA, IHC-P, IHC-F, ICC, and IF are 1:500-2000, 1:500-1000, 1:400-800, 1:400-800, 1:100-500, and 1:100-500 respectively. Antigen retrieval is needed for paraffin sections in IF. It is important to adhere strictly to these recommended dilutions for optimal results.

Immunogen

A synthetic peptide derived from human ABCG8 (566-673/673) has been conjugated with KLH. To generate content similar to this, one could write: KLH has been combined with a synthetic peptide that comes from ABCG8, specifically from the 566-673/673 region of the human sequence. This means that the resulting product is a conjugated form of the peptide and can be used for various applications.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

-20°C。/。

Storage buffer

A solution consisting of 0.01M TBS at pH7.4 enriched with 1% BSA, 0.03% Proclin300, and 50% glycerol has been formulated. This solution has been carefully arranged to provide optimal performance in the target application. By employing this solution, one can expect to achieve reliable and consistent results in their experiments. It is important to note that this solution should be handled with care and stored properly to ensure its longevity and efficacy.

Target

Background

This particular gene encodes a protein that belongs to the ATP-binding cassette (ABC) transporter superfamily. ABC proteins are responsible for transporting different kinds of molecules across cell membranes, both intra- and extra-cellularly. There are seven distinct subfamilies of ABC genes, including ABC1, GCN20, MDR/TAP, MRP, OABP, ALD, and White. The protein coded by this gene falls under the White subfamily. Its primary function is to prevent non-cholesterol sterol entry in the intestines, promote cholesterol and sterol excretion into bile, and facilitate the transport of sterols back to the intestinal lumen. This gene is found in a head-to-head orientation with ABCG5, another family member, on chromosome 2. It is primarily expressed in the liver, gallbladder, and intestine in a tissue-specific manner. Sitosterolemia patients may present with mutations in this gene, which can contribute to atherosclerosis and sterol accumulation. This information has been provided by RefSeq in July 2008.

Celluar localization

Cell membrane

UniPort

Q9H221