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WIF1 Polyclonal Antibody #abs116623

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Description

Catalog-specification	Delivery time	USD price
abs116623-50ul	In Stock	228

Overview
Other names	WIF-1
Source	Rabbit
Species Reactivity	Human,Mouse,Rat
Application	WB 1:500 - 1:2000
Immunogen	One possible rearrangement of the above content is: The recombinant fusion protein comprises a segment encoding for human WIF1 amino acids 29-220 (NP_009122.2).
Properties	Mouse trachea,Mouse heart,Mouse skeletal muscle
Properties
Purification	Affinity purification
Clonality	Polyclonal Antibody
Isotype	IgG
Stability & Storage	Store at -20℃. Avoid freeze / thaw cycles.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. To prepare buffer for PBS, add 0.02% sodium azide and 50% glycerol in a solution with a pH of 7.3. This mixture is commonly used for preservation and storage of biological samples. Remember to handle and dispose of it carefully due to its toxicity.
Target
Background	The inhibition of WNT proteins, crucial extracellular signaling molecules in embryonic development, is attributed to the protein coded by this gene. This particular protein comprises a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains. It is believed to contribute to mesoderm segmentation. In addition to these functions, this gene acts as a tumor suppressor gene and has been observed to undergo epigenetic silencing in diverse cancers.
Celluar localization	Secreted
UniPort	Q9Y5W5

Data Examples

The WIF1 antibody (abs116623) was employed for Western blot analysis of several cell line extracts at a recommended dilution of 1:1000. The secondary antibody used was HRP Goat Anti-Rabbit IgG (H+L), and it was added at a concentration of 1:10000. Each lane had 25ug of lysates/proteins while the blocking buffer consisted of 3% nonfat dry milk in TBST. Detection was performed using an ECL Enhanced Kit, and the exposure time was for 90s. Overall, the setup was set up to ensure highly accurate and reliable results.