HMGB1 Polyclonal Antibody #abs116144
Please note that the price mentioned above is just for your reference. For the detailed price, we request you to kindly get in touch with our seller Vecent. Thank you. It's important to take note that the mentioned price is only an estimate. For the exact pricing, please contact our seller...
Description
Catalog-specification | Delivery time | USD price |
abs116144-50ul | In Stock | 273 |
Please note that the price mentioned above is just for your reference. For the detailed price, we request you to kindly get in touch with our seller Vecent. Thank you. It's important to take note that the mentioned price is only an estimate. For the exact pricing, please contact our seller Vecent for more information. Thank you. We would like to inform you that the price displayed is for informative purposes only. To obtain a detailed price, please contact our seller, Vecent. Thank you. Kindly note that the price indicated is not final. For the accurate price, we suggest you reach out to our seller Vecent. Thank you.
Overview | |
Other names | HMG-1;HMG1;HMG3;SBP-1 |
Source | Rabbit |
Species Reactivity | Human;Mouse;Rat |
Application | WB 1:500 - 1:2000 |
Immunogen | A synthetic peptide has been created based on a specific sequence found in amino acids 100 to 200 of the human HMGB1 protein (NP_002119.1). This peptide is designed to closely mimic the original protein sequence and can be used in various experiments to study the function and structure of HMGB1. It is important to note that the synthetic peptide is not the actual protein, but rather a smaller fragment that can be used as a research tool. By analyzing the behavior of the synthetic peptide, researchers can gain valuable insights into the function and properties of HMGB1. |
Properties | SW480, HL-60, MCF-7, Raji, Mouse lung, Rat lung, Mouse spleen. |
Properties | |
Purification | Affinity purification |
Clonality | Polyclonal Antibody |
Isotype | IgG |
Stability & Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. |
Target | |
Background | The protein encoded by this gene is a member of the High Mobility Group-box superfamily. It acts as a non-histone, nuclear DNA-binding protein that is responsible for regulating transcription and organizing DNA within the nucleus. Its functions extend to various cellular processes including inflammation, cell differentiation, and tumor cell migration. Numerous pseudogenes derived from this gene have also been identified. Through alternative splicing, multiple transcript variants are produced, all of which encode the same protein. |
Celluar localization | 、、、-、、、、、。。，。，。，，。-，。，。，。，DNARNA，。，。，。。 |
UniPort | P09429 |
Data Examples
Western blot analysis of extracts of various cell lines, using HMGB1 antibody (abs116144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human amygdalitis using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 antibody (abs116144) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGB1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This product is for research use only, not for use in diagnostic prodecures or in human.
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