VDAC1 Antibody (Center) #abs110653

abs110653-50ul

In Stock

228

Description

Purified Rabbit Polyclonal Antibody (Pab)

Other names

VDAC-1, also known as voltage-dependent anion-selective channel protein 1, hVDAC1, outer mitochondrial membrane protein porin 1, plasmalemmal porin, porin 31HL, porin 31HM, or VDAC1, is a crucial protein found in the outer mitochondrial membrane. It plays a significant role in selective ion transport across the membrane. VDAC-1 facilitates the movement of various ions and metabolites between the cytosol and the mitochondria, thereby regulating cellular functions related to energy metabolism and apoptosis. This protein is commonly referred to as VDAC1 or VDAC due to its importance and influence on cellular processes. Its diverse array of names highlights its wide recognition and significance in research.

Source

Rabbit

Specificity

Rabbits were used to generate this VDAC1 antibody, which was immunized with a synthetic peptide between amino acids 95-124 from the central region of the human VDAC1 protein, conjugated with KLH. To produce a similar content, you could rephrase the original text like this: The VDAC1 antibody was developed by immunizing rabbits with a synthetic peptide from the central region of the human VDAC1 protein, conjugated with KLH. The peptide spanned amino acids 95-124, and the resulting antibody is highly specific for VDAC1.

Species Reactivity

Human

Predictive reaction species

B, M, Rb, R

Application

WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To ensure optimal conditions, it is advised to store the item in a refrigerator between 2-8°C for a duration of up to two weeks. However, for extended storage, it is preferable to store it at -20°C in small aliquots to prevent the occurrence of freeze-thaw cycles.

Storage buffer

The antibody is a purified polyclonal antibody that comes in a PBS solution containing 0.09% sodium azide (W/V). The antibody is produced using the Saturated Ammonium Sulfate (SAS) precipitation method and subsequently dialyzed against PBS for purification.

Research area

Cancer is a complex disease that involves abnormal cell growth and division. It is a result of disruptions in cell biology, metabolism, and signal transduction pathways. Understanding the intricate workings of these cellular processes is crucial in developing effective treatments for cancer.
In cell biology, the focus is on understanding how cells function, behave, and interact with their environment. This knowledge is crucial in deciphering the mechanisms underlying cancer development and progression. By studying the molecular and genetic basis of cancer cells, researchers can gain insights into the alterations that occur and identify potential therapeutic targets.
Metabolism plays a critical role in cancer. Cancer cells have unique metabolic characteristics that allow them to adapt to their nutrient demands and support their rapid growth. By studying the metabolic pathways that are dysregulated in cancer, scientists can uncover vulnerabilities that can be exploited for therapeutic purposes.
Neuroscience is another field that is relevant to cancer research. The nervous system plays a role in cancer progression through various mechanisms, including the release of hormones and neurotransmitters that can influence tumor growth and metastasis. Understanding the interplay between the nervous system and cancer cells can provide new avenues for treatment.
Signal transduction pathways are essential for transmitting signals within cells, controlling various cellular processes, including cell proliferation, survival, and migration. Disruptions in these pathways can contribute to cancer development. By elucidating the aberrant signaling events in cancer cells, researchers can identify potential therapeutic targets and develop drugs that can specifically target these pathways.
Overall, the fields of cancer, cell biology, metabolism, neuroscience, and signal transduction are interconnected in understanding the complex nature of cancer. By studying these areas, researchers aim to develop innovative and targeted approaches to diagnose, treat, and potentially cure cancer.

Target

Background

This channel has the ability to traverse both the outer membrane of mitochondria and the plasma membrane. Its function is twofold: on the outer mitochondrial membrane, it allows the passage of small hydrophilic molecules through diffusion; on the plasma membrane, it plays a role in regulating cell volume and initiating apoptosis. In a low or zero membrane potential environment, the channel takes on an open conformation, enabling the movement of ions. However, when the membrane potential exceeds 30-40 mV, it switches to a closed conformation that selectively allows cations to pass through. Additionally, this channel may play a role in the formation of the permeability transition pore complex (PTPC), which is responsible for the release of mitochondrial products that trigger apoptosis.

Celluar localization

Mitochondrion outer membrane. Cell membrane

UniPort

P21796

References

References

Shoshan-Barmatz,V., Biochim. Biophys. Acta 1787 (5), 421-430 (2009)
Hiller,S., Science 321 (5893), 1206-1210 (2008)