Phospho-PDK1 (Ser241) Rabbit Polyclonal Antibody#abs130646

abs130646-50ug

1-2 Weeks

201

abs130646-100ug

1-2 Weeks

301

Description

PDK1 belongs to the PKB family of AGC kinases and comprises a PH domain. Its involvement is evident in a vast range of cellular processes comprising apoptosis, differentiation and cell proliferation. The activation loop necessitates autophosphorylation for activity.

Other names

PDK1, also known as 3-phosphoinositide-dependent protein kinase 1 or hPDK1, is a protein kinase that plays a crucial role in cellular signaling. It is encoded by various aliases such as PDPK1, PDPK2, and MGC35290, to name a few.
The protein is involved in the regulation of multiple cellular processes, including metabolism, cell growth, and survival. It achieves this by phosphorylating downstream targets that participate in various intracellular pathways.
PDK1 is often referred to as a "master regulator" due to its ability to integrate different signaling pathways and relay the signals downstream. It achieves this by recognizing and binding to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the cell membrane.
PDK1 is essential for the development and function of various tissues and organs in the body. Mutations or dysregulation of PDK1 have been linked to various diseases, including cancer, diabetes, and Alzheimer's disease.
The study of PDK1 has significant implications for the development of new therapeutic strategies for these diseases, making it an active area of research in the field of molecular biology.

Source

Rabbit

Specificity

The antibody against phospho-PDK1 (Ser241) is specifically designed to recognize the phosphorylated form of PDK1 protein at Serine 241. It can accurately detect the endogenous levels of PDK1 only when it is phosphorylated at this specific site. This antibody provides a reliable means to study the phosphorylation status of PDK1 and its role in various cellular processes.

Predictive reaction species

Pig;Chicken;Sheep;Dog;Horse;Bovine;

Application

IHC 1:50-1:500, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000. Please rearrange the given information to generate a highly similar content while ensuring it is based on the original text.
The recommended dilutions for Western Blotting (WB) range from 1:500 to 1:2000. For Immunohistochemistry (IHC), the suggested dilution range is 1:50 to 1:500. In the case of ELISA (peptide), it is recommended to dilute the samples at a range of 1:20000 to 1:40000.

PDK1241，，。ChapGPT，。

Properties

1mg/ml

Purification

The highly specific antibody has been obtained from rabbit serum that has been purified through a series of chromatography techniques. The purification of the serum was carried out using both phospho- and non-phospho-peptide affinity columns to ensure that the antibody had a high level of specificity. The resulting purified antibody is highly similar to the original serum and can be used for a variety of analytical and diagnostic purposes.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid exposing the product to repeated freeze/thaw cycles as it may have a negative impact on its efficacy. Therefore, it is advisable to handle the product with care and follow the recommended storage instructions to ensure its long-term stability.

Storage buffer

This product is Rabbit IgG, dissolved in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and is stable for up to 12 months from the date of receipt. To maintain the integrity and efficacy of this product, it is important to follow the recommended storage conditions. This product is a reliable and effective tool for laboratory research in a variety of applications.

Target

Background

A master kinase known as serine/threonine kinase plays a crucial role in activating a subgroup of the AGC family of protein kinases. This kinase targets various proteins including protein kinase B, p70 and p90 ribosomal protein S6 kinases, cyclic AMP-dependent protein kinase, protein kinase C, serum and glucocorticoid-inducible kinase, p21-activated kinase-1, and protein kinase PKN. Its main function is to transduce signals from insulin by phosphorylating and activating PKB/AKT1, which propagates the signal to downstream targets that control cell proliferation and survival, glucose and amino acid uptake, and storage.
Apart from its central role in insulin signaling, the kinase also plays a crucial role in regulating TGF-beta-induced signaling, activates PPARG transcriptional activity, and promotes adipocyte differentiation. It activates the NF-kappa-B pathway and helps regulate focal adhesions by angiotensin II. Additionally, it controls the proliferation, survival, and growth of developing pancreatic cells, regulates Ca2+ entry and Ca2+-activated K+ channels of mast cells and is crucial for the motility of vascular endothelial cells.
The kinase also plays a critical role in cardiac homeostasis, serving as a dual effector for cell survival and beta-adrenergic response. It regulates the expression of key nutrient receptors during thymocyte development, mediating Notch-induced cell growth and proliferative responses. It also provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. One of its isoforms, isoform 3, is catalytically inactive.

Tissue specificity

Appears to be expressed ubiquitously. The Tyr-9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

O15530