Phospho-Survivin (Thr117) Rabbit Polyclonal Antibody#abs130645

abs130645-50ug

1-2 Weeks

201

abs130645-100ug

1-2 Weeks

301

Description

During the G2/M phase of the cell cycle, survivin emerges as a crucial apoptosis inhibitor. Its presence is intricately linked with the microtubules of the mitotic spindle. Alas, any disruption encountered in this association leads to the deprivation of apoptosis activity. It is intriguing to consider that survivin might potentially contribute to the development of neoplasia.

Other names

Apoptosis inhibitor 4, also known as API4, plays a crucial role in inhibiting cell death. It is also referred to as apoptosis inhibitor survivin or apoptosis inhibitor 4. Another name for this protein is baculoviral IAP repeat containing 5 or baculoviral IAP repeat containing protein 5. Moreover, it is known as baculoviral IAP repeat-containing protein 5 or BIRC 5. The official gene symbol for this protein is BIRC5, and it is commonly called BIRC5_HUMAN. Additionally, it is sometimes referred to as EPR 1 or IAP4. There is another variant of survivin called survivin variant 3 alpha or SVV. It is worth mentioning that TIAP represents this protein as well.

Source

Rabbit

Specificity

The Phospho-Survivin (Thr117) Antibody is able to detect the levels of endogenous Survivin protein, but only in cases when it has been phosphorylated at the specific Threonine 117 site. This indicates the importance of this particular phosphorylation event in the regulation of Survivin functionality. Therefore, if this antibody is used for detection purposes, one must ensure that the Survivin protein is indeed phosphorylated at Thr117 for accurate results.

Application

The recommended dilutions for using this antibody in various applications are as follows: for western blotting, dilute the antibody at 1:500-1:2000; for immunohistochemistry, dilute it at 1:50-1:200; for immunofluorescence or immunocytochemistry, dilute it at 1:100-1:500; and for ELISA using a peptide conjugate, dilute it at 1:20000-1:40000. It is important to note that these dilutions may vary depending on the specific experimental conditions and background signals. Therefore, optimization may be necessary for each individual experiment.

A peptide that has been synthesized from the human protein known as Survivin is focused around the area where the amino acid Threonine 117 undergoes phosphorylation. I can create a paragraph with a similar message by rephrasing the original content. For instance: A peptide has been developed by utilizing human Survivin as the source material. This peptide is concentrated on the phosphorylation site of Threonine 117, which is a crucial amino acid residue in the Survivin protein. This synthesized peptide is designed to be structurally similar to the native protein, ensuring that it can perform specific functions related to Survivin.

Properties

1mg/ml

Purification

，。

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

This Rabbit IgG solution is formulated in a phosphate buffered saline with a pH of 7.4, 150mM NaCl, and 0.02% sodium azide. It also contains 50% glycerol and should be stored at -20°C. This solution is stable for a year from the date of receipt, ensuring that you can rely on its quality for laboratory applications. With its highly similar content, this Rabbit IgG solution is an effective tool for research purposes and experiments.

Target

Background

This multitasking protein serves dual roles in promoting cell proliferation and preventing apoptosis. It is part of a crucial chromosome passage protein complex (CPC) that plays an essential role in chromosome alignment and segregation during mitosis and cytokinesis. The protein is responsible for directing the CPC to different locations from the inner centromere during prometaphase to midbody during cytokinesis, thus participating in the organization of the center spindle by associating with polymerized microtubules.
In addition, this protein is involved in the recruitment of CPC to centromeres during early mitosis, and it plays a crucial role in mitotic spindle formation during cell division by serving as a physical scaffold that helps deliver TPX2 to microtubules.
Moreover, this protein may counteract a default induction of apoptosis in G2/M phase and inhibit CASP3 and CASP7 in the cell. It is also involved in repressing STAT3 transactivation of target gene promoters.
Although isoform 2 and 3 do not appear to play vital roles in mitosis, isoform 3 shows a marked reduction in its anti-apoptotic effects when compared to the displayed wild-type isoform.
Taken together, this multitasking protein plays a crucial role in cell division and proliferation, preventing apoptosis, and regulating various cellular processes essential for overall cellular health.

Tissue specificity

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain (PubMed:10626797). Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas (PubMed:14741722, PubMed:16329164). Also expressed in various renal cell carcinoma cell lines (PubMed:10626797). Expressed in cochlea including the organ of Corti, the lateral wall, the interdental cells of the Limbus as well as in Schwann cells and cells of the cochlear nerve and the spiral ganglions (at protein level). Not expressed in cells of the inner and outer sulcus or the Reissner's membrane (at protein level) (PubMed:21364656, PubMed:20627126).

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

O15392