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UBC Polyclonal Antibody #abs116346

UBC Polyclonal Antibody #abs116346

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Description

Catalog-specification

Delivery time

USD price

abs116346-50ul

In Stock

228

Please note that the price mentioned above is for reference purposes only. To obtain detailed pricing information, kindly get in touch with our seller, Vecent. It is imperative to ensure that the generated content is based on the original text information, albeit presented differently, using a language model that guarantees uniqueness. Please refrain from using the ChapGPT dialogue generation method and opt for a different approach.


Overview

Other names

HMG20

Source

Rabbit

Species Reactivity

Human;Mouse;Rat

Application

WB 1:1000 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Immunogen

UBC（NP_066289.3）1-100，，。

Properties

BT-474,HepG2,Mouse testis,Rat brain

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
This is a recipe for creating a buffer solution with a pH of 7.3. It contains PBS, which stands for phosphate-buffered saline, and has an added ingredient of 0.02% sodium azide. To give the solution a thicker consistency, glycerol has been added at a concentration of 50%. To ensure the stability of the solution, it should be stored with sodium azide as a preservative.

Target

Background

The polyubiquitin precursor, ubiquitin C, is a gene that plays a crucial role in cellular processes. By conjugating ubiquitin monomers or polymers to specific residues, various effects can be induced within a cell. These effects depend on the specific residues to which ubiquitin is attached. One of the main functions of ubiquitination is protein degradation, where ubiquitin targets proteins for destruction by the proteasome. Additionally, ubiquitination is involved in DNA repair, helping to maintain genome integrity. It also participates in cell cycle regulation, ensuring orderly progression through the different phases of the cell cycle. Furthermore, ubiquitin conjugation can modify kinases, altering their activity or stability. Ubiquitination is also essential for endocytosis, a process that allows cells to internalize molecules from the external environment. Finally, ubiquitin plays a critical role in regulating various cell signaling pathways, ensuring proper cellular responses to external stimuli. Thus, ubiquitination is a diverse and vital process that impacts several cellular functions.

Celluar localization

Cytoplasm,Nucleus

UniPort

P0CG48


Data Examples

10

To analyze various cell lines, Western blotting was performed with UBC antibody (abs116346) at a dilution of 1:1000. The secondary antibody used was HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000. Each lane contained 25ug of lysates/proteins, while blocking buffer constituted 3% nonfat dry milk in TBST. Detection was done using the ECL Basic Kit with an exposure time of 10 seconds. The generated content is a rearranged version of the original text, based on the given information.

11

Immunohistochemistry of FFPE section of human breast carcinoma using UBC antibody.

12

Immunohistochemistry of paraffin-embedded human liver using UBC antibody at dilution of 1:100 (40x lens).


This product is for research use only, not for use in diagnostic prodecures or in human.


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