Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-TOP2B Polyclonal Antibody #abs130219

Please be advised that the price provided is solely for your reference. For a detailed price, kindly reach out to our seller Vecent. We recommend contacting Vecent for further information on pricing. Please note that the generated content should be significantly different from the original text...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs130219-50ug	1-2 Weeks	201
abs130219-100ug	1-2 Weeks	301

Overview
Description	TOP2B is responsible for controlling the topological states of DNA through the transient breakage and subsequent rejoining of DNA strands. As a type II topoisomerase family member, it is able to create double-strand breaks which play a crucial role in various biological processes. Moreover, TOP2B indirectly plays a role in vitamin D-coupled transcription regulation by associating with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). This complex is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In summary, TOP2B is a multifaceted protein that is essential for maintaining the integrity of DNA and regulating gene expression. Its crucial role in various biological processes highlights its significance in the functioning of living organisms.
Other names	DNA topoisomerase II beta, also known as TOP2B or Topo II beta, is an important enzyme involved in DNA replication and chromosome segregation. It plays a crucial role in maintaining the integrity of the genome by regulating DNA supercoiling and untangling DNA during various cellular processes. This enzyme is encoded by the TOP2B gene and its isoform, Antigen MLAA 44, is associated with U937 cells. The beta isozyme of DNA topoisomerase II, also referred to as DNA topoisomerase 2-beta or DNA topoisomerase II beta, is a 180 kDa protein. It is structurally and functionally similar to the alpha isozyme, DNA topoisomerase II alpha. Together, these enzymes help in the proper packaging and organization of DNA in the cell nucleus. TOP2B is primarily expressed in dividing cells and is essential for processes like DNA replication, transcription, and recombination. It catalyzes the transient double-stranded DNA breaks, allowing DNA strands to pass through each other and relieve the torsional stress generated during DNA replication and transcription. Mutations in the TOP2B gene have been associated with various diseases, including cancer and developmental disorders. Understanding the mechanisms of action and regulation of DNA topoisomerase II beta is crucial for developing targeted therapies against these diseases.
Source	Rabbit
Specificity	The TOP2B antibody is capable of detecting total TOP2B present in the body naturally. This indicates that the antibody can identify and bind to the endogenous levels of TOP2B without any external interference. A highly similar content could be generated by rephrasing the original information as follows: The inherent levels of TOP2B in the body can be recognized and measured effectively with the use of the TOP2B antibody without any external interference. This indicates the potential of the antibody to detect and bind to the total endogenous TOP2B levels in the system.
Species Reactivity	Human;Mouse
Predictive reaction species	Sheep;Dog;
Application	：Western: 1:500~1:3000，（IHC）: 1:50~1:200，/（IF/ICC）: 1:100-1:500，（ELISA，）: 1:20000-1:40000。，，ChapGPT。
Immunogen	A synthesized peptide derived from human TOP2B.
Properties
Concentration	1mg/ml
Purification	SulfoLink™ Coupling Resin was utilized in the peptide affinity chromatography purification process of the antiserum. The resulting product has been highly purified and is suitable for further use in various biological assays and experiments.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the product's quality, it is recommended to keep it stored at a temperature of -20 °C and avoid exposing it to repeated cycles of freezing and thawing. This storage condition should be maintained for up to one year.
Storage buffer	The storage conditions for Rabbit IgG include keeping it in phosphate buffered saline with a pH of 7.4 and containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20 °C and has a stability period of 12 months starting from the date of receipt.
Target
Background	Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Celluar localization	Cytosol;Nucleus;
UniPort	Q02880