Rabbit Anti-Ki67 Polyclonal Antibody #abs130135

abs130135-50ug

In Stock

201

abs130135-100ug

In Stock

301

Description

The protein KI-67 is believed to act as a marker for cells that are undergoing proliferation, and it plays a role in the compacting of chromatin. However, researchers have found that its expression can be changed in various tumor types, such as osteosarcomas, histiocytomas, prostate cancer, breast cancer, and esophageal cancer. Additionally, KI-67 has been found to be mutated in colon cancer, cervical cancer, and lung cancer.

Other names

The monoclonal antibody Ki-67 identifies an antigen known as Ki-67. This antigen, also referred to as KI-67 or Ki67, is associated with proliferation and serves as a marker of cell growth. It is encoded by the MKI67 gene and is also known by its alternative name, protein phosphatase 1 regulatory subunit 105 (PPP1R105). The antigen KI-67 is detected using the MIB 1 antibody and is commonly used as a proliferation marker in various research and diagnostic applications. Additionally, it is linked to the RP11-380J17.2 gene. Overall, the antigen identified by monoclonal antibody Ki-67, or Ki67, plays a crucial role in monitoring and studying cell proliferation.

Source

Rabbit

Specificity

Ki67 Antibody detects endogenous levels of Ki67.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Rabbit;Bovine;

Application

The recommended dilutions for various applications of the primary antibody are as follows: Western blotting (WB) requires a dilution range of 1:500-1:2000, while immunohistochemistry (IHC) requires a dilution range of 1:50-1:200. For immunofluorescence (IF/ICC), the suggested dilution range is 1:100-1:500. Finally, for ELISA(peptide), a dilution range of 1:20000-1:40000 is required. It is important to note that these dilutions may vary based on specific experimental conditions and it is recommended to conduct a titration for optimal results.

Immunogen

A synthesized peptide derived from human Ki67.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the longevity of the product, it is advisable to store it at a temperature of -20°C for up to one year. However, it is important to note that repeated freeze/thaw cycles should be avoided as it may compromise the quality of the product. Hence, it is recommended to take necessary precautions to maintain the ideal storage conditions for the product.

Storage buffer

The storage solution for Rabbit IgG includes phosphate buffered saline, pH 7.4, along with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to keep the IgG at a temperature of -20 °C for optimal stability, ensuring a shelf life of 12 months from the date of receipt.

Target

Background

The protein discussed here is responsible for keeping individual mitotic chromosomes dispersed in the cytoplasm after the nuclear envelope disassembles. It forms a barrier on the surface of the chromosomes, known as the perichromosomal layer, covering a significant portion of their surface. This barrier prevents the chromosomes from collapsing into a single chromatin mass. Its mechanism involves both steric and electrostatic charge interactions. Due to its high net electrical charge, the protein acts as a surfactant, allowing for independent chromosome motility and dispersing them throughout the cytoplasm. It has a binding affinity for DNA, particularly supercoiled and AT-rich DNA. However, it does not play a role in the internal structure of mitotic chromosomes. While it is unclear whether the protein directly contributes to chromatin organization, it may have an indirect effect resulting from its function in maintaining dispersed mitotic chromosomes.

Tissue specificity

Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).

Posttranslational modification

Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.

Celluar localization

Nucleus;

UniPort

P46013