Rabbit Anti-APAF1 Polyclonal Antibody #abs130054

abs130054-50ug

1-2 Weeks

201

abs130054-100ug

1-2 Weeks

301

Description

Apaf-1, also known as apoptotic protease-activating factor 1, is a protein that plays a critical role in inducing apoptosis or programmed cell death. It mediates the cytochrome c-dependent activation of pro-caspase-9, which ultimately leads to the activation of caspase-3 and the execution of apoptosis. It is important to note that this process requires ATP.
There are six isoforms of Apaf-1 that are produced through alternative splicing of the gene. However, isoform 6 is less effective in inducing apoptosis than the other isoforms.
The activation of Apaf-1 is induced by E2F and p53 in apoptotic neurons. When cytochrome c binds to Apaf-1 and ATP, it leads to the oligomerization of Apaf-1. Pro-caspase-9 then binds to the Apaf-1 oligomer via their respective NH2-terminal CARD domains. This binding leads to the autocatalytic activation of pro-caspase-9 and subsequent release of mature caspase-9 from the complex.
Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. The activation of caspase-3, which is a key player in the execution of apoptosis, ultimately leads to cell death.
It is also worth mentioning that Apaf-1 interacts with APIP, an apoptotic protein, which further regulates the apoptotic process. Overall, the crucial role of Apaf-1 in regulating apoptosis makes it an important target for therapeutic interventions in various diseases such as cancer.

Other names

Apaf-1, also known as apoptotic protease activating factor 1, is a crucial factor in initiating the mitochondrial-dependent apoptosis pathway. It is encoded by the APAF1 gene and is a key mediator of programmed cell death. Apaf-1 binds and activates caspase-9, initiating the downstream cascade of events leading to apoptosis. Its role in cell death regulation has made Apaf-1 an important target for therapeutic intervention in various diseases, including cancer. The protein structure of Apaf-1 consists of multiple domains, including a CARD domain and WD repeat domains. Mutations in the APAF1 gene have been associated with multiple diseases, including lymphoma and leukemia. Thus, understanding the function and regulation of Apaf-1 has profound implications not only in basic biology but also in clinical applications.

Source

Rabbit

Specificity

The endogenous levels of total APAF1 can be detected using the APAF1 antibody. This antibody is specifically designed to target APAF1 and is capable of accurately detecting its presence. By targeting total APAF1, it allows for a comprehensive analysis of the protein and its various forms. This antibody is a valuable tool for researchers studying APAF1 and its functions in various biological processes. With its high sensitivity and specificity, it provides reliable results and contributes to a better understanding of APAF1's role in cellular activities.

Species Reactivity

Human

Application

We recommend the following dilution factors for different applications: 1:500~1:3000 for Western blotting (WB), 1:50~1:200 for immunohistochemistry (IHC), 1:100-1:500 for immunofluorescence/immunocytochemistry (IF/ICC), and 1:20000-1:40000 for ELISA using peptide antigens. These dilutions have been optimized for optimal signal-to-noise ratio and reliable detection of the target protein.

Immunogen

A synthesized peptide derived from human APAF1.

Properties

Concentration

1mg/ml

Purification

The purification of the antiserum involved the use of SulfoLink™ Coupling Resin for peptide affinity chromatography. This method facilitated the separation of the desired immunoglobulins from other unwanted components. The SulfoLink™ resin allowed for a highly specific binding of the target peptides to the resin, resulting in a highly pure antiserum product. Overall, the peptide affinity chromatography process was efficient and effective in purifying the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is important to store it at a temperature of -20 °C for a duration of one year. It is also advised to prevent exposing the product to repetitive cycles of freezing and thawing. By following these guidelines, the quality of the product can be maintained for an extended period.

Storage buffer

The storage condition for Rabbit IgG is a phosphate buffered saline solution with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature is -20 °C, and the product can be stored for up to 12 months from the date of receipt.

Target

Background

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Tissue specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Celluar localization

Cytosol;Extracellular region or secreted;Nucleus;

UniPort

O14727