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Rabbit Anti-ARC Polyclonal Antibody #abs130055

Please note that the price provided above is only for your reference. If you require detailed pricing information, kindly get in touch with our seller, Vecent. Data Examples Western blot analysis on HeLa cell lysate using ARC Antibody This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs130055-50ug	1-2 Weeks	201
abs130055-100ug	1-2 Weeks	301

Please note that the price provided above is only for your reference. If you require detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
Description	According to research, ARC Isoform 1 appears to play a role in RNA splicing and interacts with TFPT. It has been observed that this isoform has the ability to cluster together and bind to SFRS9/SRp30C. Additionally, it also interacts with NPM1. On the other hand, Isoform 2 has a completely different function. It binds to CASP2/caspase-2 and CASP8/caspase-8, effectively preventing the activity of caspase-8. It is noteworthy that humans produce two isoforms of this protein, with the difference being due to alternative splicing.
Other names	The protein known as Nucleolar protein 3, or Nol3, is a muscle-enriched cytoplasmic protein that has been shown to act as an apoptosis repressor with CARD domain. Its other names include ARC, Muscle enriched cytoplasmic protein, MYC, MYP, and NOP. Its molecular weight is approximately 30 kDa and it is primarily found in the nucleoli of cells. Nol3 is an important regulatory protein in cell death and survival pathways. It is a potent inhibitor of apoptosis, which allows cells to survive during times of stress. Nol3 achieves this by blocking the activation of caspases, which are enzymes that play a key role in apoptotic cell death. The regulation of Nol3 is complex and involves various signaling pathways. It has been found to be overexpressed in many cancers, where it promotes tumor cell survival. On the other hand, some studies suggest that Nol3 may also have a role in promoting cell death under certain conditions. Despite its importance in cell biology, much is still unknown about the exact mechanism of Nol3 function. However, continued research into the protein's role in apoptosis and other cellular processes is likely to yield valuable insights into its biological significance.
Source	Rabbit
Specificity	The detection of endogenous levels of total ARC can be achieved using the ARC antibody.
Species Reactivity	Human
Application	The recommended dilutions for Western blotting (WB) range from 1:500 to 1:3000. For immunohistochemistry (IHC), the dilutions typically range from 1:50 to 1:200. If you are performing immunofluorescence or immunocytochemistry (IF/ICC), the suggested dilutions are between 1:100 and 1:500. When using ELISA with peptide antigens, it is recommended to dilute the samples between 1:20000 and 1:40000. These dilution ranges are crucial for achieving optimal results in each respective assay.
Immunogen	A synthesized peptide derived from human ARC.
Properties
Concentration	1mg/ml
Purification	A process called peptide affinity chromatography was employed to purify the antiserum, and it made use of SulfoLink™ Coupling Resin. Let me know if you need any further information.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to note that the product should not be subjected to repeated cycles of freezing and thawing as this can impact its effectiveness. Therefore, it is necessary to take appropriate measures to prevent any changes in the temperature of the product during storage.
Storage buffer	This Rabbit IgG solution is prepared in a pH 7.4 phosphate buffered saline containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20°C for optimal stability, and can be safely used for up to 12 months from the date of receipt.
Target
Background	Isoform 1: May be involved in RNA splicing.
Tissue specificity	Expressed at high levels in heart and skeletal muscle, while found at lower levels in placenta, liver, kidney, and pancreas.
Posttranslational modification	Phosphorylation at Thr-149 is required for its antiapoptotic effect by blocking death-inducing signaling complex death-inducing signaling complex (DISC) activity through the control of interaction with CASP8. Phosphorylation at Thr-149 results in translocation to mitochondria and this translocation enables the binding to CASP8. Dephosphorylated at Thr-149 by calcineurin; doesn't inhibit the association between FADD and CASP8 and the consequent apoptosis.Polyubiquitinated by MDM2; promoting proteasomal-dependent degradation in response to apoptotic stimuli.
Celluar localization	Cytosol;Endoplasmic reticulum;Mitochondrion;Nucleus;
UniPort	O60936