Rabbit Anti-Bak Polyclonal Antibody #abs130056

abs130056-50ug

1-2 Weeks

201

abs130056-100ug

1-2 Weeks

301

Description

Bak1 is a protein that is involved in programmed cell death, or apoptosis. This process is triggered by a stimulus, and Bak1 binds to proteins like BCL2 or E1B 19k to accelerate apoptosis. Interestingly, low levels of zinc ions can actually inhibit this process. Bak1 belongs to the Bcl-2 family of proteins and interacts with other proteins like BCL2A1, BCL2, and BCL2L1 isoform Bcl-X(L). It can form homodimers and heterodimers with these proteins, with the formation of the homodimer being dependent on zinc. Bak1 even interacts with proteins from viruses like myxoma virus protein M11L.

Other names

Bak, also known as Apoptosis regulator BAK and Bcl-2 homologous antagonist/killer, is a crucial protein involved in regulating cellular apoptosis. It is also referred to as BAK_HUMAN, Bak1, Bak NT, BAK like, and BCL2L7.
Bak plays a significant role in apoptosis, or programmed cell death, by promoting cell death and inhibiting cell survival. It acts as an antagonist for Bcl-2 family proteins, particularly Bcl-2-like 7 protein and Bcl-2 homologous antagonist/killer, to induce cell death. Its pro-apoptotic function makes it a vital player in maintaining cell homeostasis and eliminating damaged or unnecessary cells.
Researchers have identified Bak as a Bcl-2-like protein 7 and a BCL2 antagonist/killer 1. It is also known by other names such as Bcl2 like 7 Protein, Bcl2-L-7, Cell death inhibitor 1, and NBak. Additionally, it has been assigned aliases like MGC117255 and MGC3887.
Understanding the mechanisms and regulation of Bak is essential in unraveling the intricate processes of apoptosis and potential therapeutic interventions for diseases where aberrant cell death or survival occurs. The study of Bak provides invaluable insights into the delicate balance between cell survival and programmed cell death, ultimately influencing various physiological and pathological conditions.

Source

Rabbit

Specificity

The Bak antibody is able to recognize and bind to total Bak at its natural levels within the body.

Species Reactivity

Human;Mouse

Predictive reaction species

Horse

Application

Western blot (WB) is typically performed at dilutions ranging from 1:500 to 1:3000. For immunohistochemistry (IHC), the recommended dilutions are between 1:50 and 1:200. In the case of immunofluorescence (IF/ICC), dilutions of 1:100 to 1:500 are commonly used. When it comes to ELISA with peptide antigens, the suggested dilutions range from 1:20000 to 1:40000. It is important to adhere to these dilution ranges to ensure accurate and reliable results in these assays.

Immunogen

A synthesized peptide derived from human Bak.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography purification of the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended to keep the product in a freezer at -20 °C for up to one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this can compromise its quality. By following these storage guidelines, you can maintain the integrity and effectiveness of the product for longer periods of time.

Storage buffer

：IgGpH 7.4，150mM NaCl、0.02%50%。-20°C。，12。

Target

Background

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.

Tissue specificity

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Celluar localization

Cytosol;Endoplasmic reticulum;Mitochondrion;

UniPort

Q16611