CDC42 Polyclonal Antibody #abs115467

abs115467-50ul

In Stock

228

Other names

CDC42Hs;G25K;TKS

Source

Rabbit

Species Reactivity

Human,Mouse,Rat

Application

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Immunogen

A highly similar recombinant fusion protein can be created by incorporating a specific sequence from human CDC42 (NP_001782.1). This sequence encompasses amino acids 25 to 191 and forms an essential part of the protein.

Properties

Jurkat,Mouse brain

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
A solution of PBS containing 0.02% sodium azide and 50% glycerol has a pH of 7.3. This formulation is commonly used as a buffer for various biological applications. The addition of sodium azide helps to prevent bacterial growth, while glycerol acts as a cryoprotectant to protect sensitive samples during freezing and thawing. The pH of the buffer is important for maintaining the stability of proteins and other biomolecules in the solution. Overall, this buffer provides a reliable and effective means for maintaining the integrity of biological samples.

Target

Background

This gene encodes a small GTPase belonging to the Rho-subfamily, which plays a crucial role in regulating various cellular functions like cell morphology, migration, endocytosis, and cell cycle progression. It shares a high similarity with Saccharomyces cerevisiae Cdc 42 and can effectively complement the yeast cdc42-1 mutant. Interestingly, the oncogene Dbl has been found to specifically stimulate the release of GDP from this protein. By directly binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), this protein can potentially control actin polymerization and subsequently activate the Arp2/3 complex. Multiple transcript variants result from alternative splicing of this gene. Additionally, pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8, and 20.

Celluar localization

The cell membrane is a crucial component of cells, serving as a barrier between the cell and its environment. Within the cell, the cytoplasm fills the space between the membrane and the various organelles. The cytoplasmic side of the membrane is where membrane-bound proteins are anchored, often using lipid-anchors. The cytoskeleton and microtubule organizing center, which includes the centrosome, play important roles in cell division, with the spindle and midbody forming during mitosis.

UniPort

P60953