Phospho-MAPKAPK5(Thr182) Rabbit Polyclonal Antibody #abs130026

abs130026-50ug

1-2 Weeks

201

abs130026-100ug

1-2 Weeks

301

Description

This gene encodes a member of the serine/threonine kinase family, with its protein product playing a crucial role in the cellular response to stress and inflammation. Activation of this kinase is triggered by phosphorylation via various MAP kinases such as MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. HSP27, a heat shock protein, is one of the many proteins that can be phosphorylated by this kinase in vitro. This gene has two distinct isoforms resulting from alternative splicing of transcripts.

Other names

Protein kinase 5, also known as MAP kinase-activated protein kinase 5 or MAPKAPK-5, is a crucial enzyme involved in cellular signaling pathways. It plays a significant role in the regulation and activation of mitogen-activated protein kinase (MAP kinase). In humans, this protein is encoded by the MAPK5 gene.
MAPKAPK-5, also referred to as p38-regulated/activated protein kinase or PRAK, participates in diverse biological processes, including cell proliferation, differentiation, and survival. Its activation is triggered by MAP kinase and it subsequently phosphorylates various downstream targets, thereby influencing cellular responses.
The function of MAPKAPK-5 is essential in numerous physiological and pathological conditions. It has been implicated in the modulation of immune responses, stress-induced apoptosis, and regulation of cellular motility. By studying this protein, researchers aim to gain a better understanding of its molecular mechanisms and potential therapeutic applications.
In conclusion, MAP kinase-activated protein kinase 5, or MAPKAPK-5, is a critical enzyme involved in cellular signaling pathways. Its activation and phosphorylation of downstream targets regulate various cellular processes, making it a significant player in physiological and pathological conditions. Further research on this protein may provide insights into its therapeutic potential.

Source

Rabbit

Specificity

The presence of phosphorylated MAPKAPK5 at Threonine 182 can be detected by the Phospho-MAPKAPK5(Thr182) Antibody. This antibody specifically targets the endogenous levels of MAPKAPK5 that are phosphorylated at Threonine 182. To obtain accurate results, it is essential to use this antibody, as it is highly sensitive to this specific phosphorylation site.

Species Reactivity

Human;Mouse

Application

The recommended dilutions for Western blotting (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the suggested dilutions are 1:50 to 1:200. As for ELISA using peptides, the appropriate dilutions are 1:20000 to 1:40000. It is important to adhere to these dilutions to ensure accurate and reliable results.

Immunogen

A peptide that has been synthesized from the human MAPKAPK5 protein sequence, specifically targeting the region around Threonine 182, where phosphorylation occurs. The synthesized peptide is highly similar to the original sequence, ensuring that its properties are consistent with those of the native protein. This peptide may have important applications in studies related to cellular signaling pathways and protein interactions, as well as potential therapeutic uses.

Properties

Concentration

1mg/ml

Purification

The antibody was obtained by purifying rabbit serum through sequential chromatography. The purification process involved passing the serum through both phospho- and non-phospho-peptide affinity columns to isolate the desired antibody.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain optimum storage conditions, it is recommended to store the product at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to repeated cycles of freezing and thawing.

Storage buffer

The Rabbit IgG solution is composed of phosphate buffered saline with a pH of 7.4, supplemented with 150mM of NaCl, 0.02% of sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20 °C, and it is stable for up to 12 months from the date of receipt. The solution is ideal for use in a variety of biological applications and can be easily incorporated into research protocols. With its optimal composition and long shelf life, the Rabbit IgG solution is a reliable choice for any laboratory looking for high-quality reagents.

Target

Background

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.

Tissue specificity

Expressed ubiquitously.

Posttranslational modification

Phosphorylated on Thr-182 ERK3/MAPK6 or ERK4/MAPK4; which is the regulatory phosphorylation site and is located on the T-loop/loop 12, leading to activation. Phosphorylation at Thr-182 by p38-alpha/MAPK14, p38-beta/MAPK11 is subject to debate. Phosphorylated at Ser-115 by PKA/PRKACA, leading to localization to the cytoplasm. Autophosphorylated (By similarity).

Celluar localization

Cytosol;Nucleus;

UniPort

Q8IW41