Bax Antibody #abs130057

abs130057-50ug

1-2周

￥1100.00

abs130057-100ug

现货

￥1650.00

Description

Bax is a protein that plays a crucial role in the process of programmed cell death. It achieves this function by binding to certain proteins and impeding their activity. One such protein is BCL2, which is a potent inhibitor of apoptosis. Bax neutralizes the effect of BCL2, thereby enabling the cell to initiate the apoptotic process. It does this by prompting the release of cytochrome c and activating a protein called CASP3, both of which are critical components in the apoptotic pathway.
Bax belongs to the Bcl-2 family of proteins and exists primarily as a homodimer. However, it can also form heterodimers with other proteins in this family, such as BCL2L1 isoform Bcl-X(L), MCL1, and A1. Bax also interacts with various other proteins, such as SH3GLB1, HN, SFN, and YWHAZ. The latter two proteins bind to Bax in the cytoplasm and under stressful conditions, their phosphorylation via JNK triggers the release of Bax to mitochondria.
Interestingly, there are several different isoforms of Bax produced by alternative splicing. Isoform Sigma, for example, interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Despite these variations in structure, all forms of Bax retain their essential function in promoting apoptosis.

Other names

BAX, also known as apoptosis regulator BAX or Bax-protein, is a protein that plays an important role in regulating cell death. It is a member of the Bcl-2 family of proteins and is involved in the mitochondrial pathway of apoptosis.
BAX is encoded by the BCL2L4 gene and is also known as Bcl-2-like protein 4. It has several isoforms, including Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, and the membrane isoform alpha.
As an apoptosis regulator, BAX is involved in promoting programmed cell death in response to various stress stimuli, such as DNA damage, radiation, and viral infection. It does this by binding and permeabilizing the mitochondrial outer membrane, leading to the release of cytochrome c and activation of downstream apoptotic signaling pathways.
However, BAX also has a role in promoting cell survival. It can inhibit the anti-apoptotic activity of other Bcl-2 family members and protect cells from death in response to certain stress stimuli.
Overall, BAX is a complex and multifunctional protein with important roles in regulating cell fate. Its intricate interactions with other Bcl-2 family members contribute to the delicate balance between cell survival and death.

Source

Rabbit

Specificity

The detection of endogenous levels of total Bax is possible through the use of Bax antibody. By rearranging the original content, the following statement is generated: "Total Bax can be efficiently detected using the Bax antibody, enabling the measurement of its endogenous levels."

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Rabbit;Pig;Dog;Bovine;Horse

Application

The recommended dilution range for this antibody is 1:500 to 1:3000 for Western blotting, 1:50 to 1:200 for immunohistochemistry, 1:200 for immunofluorescence, and 1:20000-1:40000 for ELISA using peptide as the antigen. It is important to follow these guidelines to achieve optimal results with this antibody.

Immunogen

A synthesized peptide derived from human Bax.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure preservation, it is recommended to store at -20 °C for a duration of one year. It is crucial to avoid subjecting the item to repeated freezing and thawing cycles.

Storage buffer

The storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4 and a concentration of 150mM NaCl. Additionally, it is recommended to add glycerol for stabilization. The stability of the product is guaranteed for a period of 12 months starting from the date of receipt.

Target

Background

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Tissue specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Celluar localization

Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Mitochondrion;Nucleus;

UniPort

Q07812