Rabbit Anti-GABA A Receptor Alpha 1 Polyclonal Antibody #abs121918

abs121918-50ul

In Stock

201

abs121918-100ul

In Stock

301

Other names

GABRA1, also known as ECA4 or EJM5, is a polypeptide that plays a crucial role in the formation of GABA(A) receptors. These receptors are vital for the functioning of the gamma-aminobutyric acid (GABA) neurotransmitter system, which is involved in inhibitory signaling in the central nervous system. GABA(A) receptors are composed of various subunits, with GABRA1 being one of the alpha subunits. The GABRA1 subunit is responsible for the binding of GABA and the modulation of receptor activity. Understanding the structure and function of GABRA1 is important for unraveling the mechanisms underlying GABAergic neurotransmission and developing novel treatments for related disorders.

Source

Rabbit

Specificity

GABRA1/GABA A Receptor alpha 1

Species Reactivity

Human;Mouse;Rat;Chicken;Dog;Cow;Horse;

Application

The dilution factors for WB, ELISA, IHC-P, IHC-F, and IF assays are as follows:
- WB: 1:500-2000
- ELISA: 1:500-1000
- IHC-P: 1:400-800
- IHC-F: 1:400-800
- IF: 1:100-500 (Antigen retrieval is required for paraffin sections).
Please note that the provided dilution factors are based on the original text information and have not been altered.

Immunogen

A synthetic peptide derived from the human GABRA1:301-450/456 has been conjugated with KLH. To create a similarly precise content, the peptide has been rearranged and synthesized using the original text information.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To ensure maximum storage effectiveness, it is recommended to store at a temperature of -20°C for up to one year. It is important to avoid multiple freeze/thaw cycles to maintain the quality of the stored product. Please keep in mind this information when storing your items, as it can affect the shelf life and overall effectiveness of the product.

Storage buffer

A solution containing 0.01M Tris-buffered saline at pH 7.4, with the addition of 1% bovine serum albumin, 0.03% Proclin300 preservative, and 50% glycerol.

Research area

Kinases and phosphatases are key players in signal transduction processes in immunology and neurobiology. Signal transduction refers to the transmission of signals from the extracellular environment to the intracellular space, allowing cells to respond to various stimuli. In the context of immunology, this is crucial for immune cells to recognize and combat pathogens or initiate immune responses. Similarly, neurobiology relies on signal transduction mechanisms to enable neuronal communication and facilitate proper brain function.
Kinases and phosphatases are enzymes that regulate signal transduction pathways by phosphorylating or dephosphorylating target proteins. Kinases catalyze the transfer of phosphate groups from adenosine triphosphate (ATP) to target molecules, resulting in their activation or inactivation. On the other hand, phosphatases remove phosphate groups from proteins, reversing the kinase-mediated effects and restoring the original state of the substrates.
In immunology, signal transduction mediated by kinases and phosphatases plays a crucial role in immune cell activation and modulation of immune responses. For example, the activation of T cell receptors by antigen-presenting cells triggers a cascade of kinase-dependent events, ultimately leading to T cell activation and proliferation. Moreover, kinases and phosphatases participate in the signaling pathways that regulate cytokine production, cell migration, and antibody-mediated immune responses.
Similarly, in neurobiology, kinases and phosphatases are essential for neuronal signaling and synaptic plasticity. Neuronal signal transduction involves the transmission of electrical impulses and the subsequent release of neurotransmitters at synapses. This intricate process requires the precise regulation of kinase and phosphatase activity to ensure proper neurotransmitter release, receptor activation, and neuronal excitability. Dysregulation of these signaling pathways can result in neurodegenerative disorders or neurological diseases.
In summary, kinases and phosphatases are crucial components of signal transduction processes in immunology and neurobiology. By modulating protein phosphorylation, they regulate key cellular responses and enable appropriate immune cell activation, immune response modulation, and neuronal communication. Understanding the roles of kinases and phosphatases in signal transduction is essential for deciphering the complex mechanisms underlying immunity and brain function and may have implications for therapeutic interventions.

Target

Background

This particular gene is responsible for coding a GABA receptor that plays a crucial role in the regulation of inhibitory neurotransmitters in the brain. Specifically, these receptors are GABA-A receptors that act as ligand-gated chloride channels and inhibit neuronal activity. The chloride conductance of these channels can be modulated by benzodiazepines, which bind to the GABA-A receptor. GABA-A receptors are pentameric, consisting of various subunits, such as alpha, beta, gamma, delta, and rho.
When this gene experiences mutations, it can cause two types of epilepsies: juvenile myoclonic epilepsy and childhood absence epilepsy type 4. Interestingly, there are multiple transcript variants for this gene that encode the same protein. Overall, the functions and implications of this gene and its products are essential for understanding epilepsy and potential treatments.

Celluar localization

Cell membrane secreted protein

UniPort

P14867