IGF2BP1 Antibody (C-term) #abs100689

abs100689-50ul

In Stock

228

Description

Antibody purification using peptide affinity has been performed to obtain a rabbit polyclonal antibody (Pab). The antibody was purified, and a specific peptide was used for antigen binding. This technique allows for efficient isolation and enrichment of the desired antibody, ensuring high specificity and sensitivity in downstream applications. The resulting Pab can be used for various research purposes, such as immunoblotting, immunohistochemistry, or immunoprecipitation, to detect and study specific target proteins.

Other names

Coding region determinant-binding protein, also known as CRD-BP, is an important mRNA-binding protein that plays a crucial role in regulating gene expression. It is also commonly referred to as insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), IMP-1, IMP1, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, or ZBP-1.
IGF2BP1 is a multifunctional protein that is involved in various cellular processes, such as mRNA localization, stabilization, and translation. It binds to specific RNA sequences, called zipcode elements, to transport mRNAs to their designated cellular locations and prevent their degradation. Additionally, IGF2BP1 has been found to regulate the expression of a variety of genes, including growth factors and oncogenes, suggesting that it is involved in cell growth, differentiation, and cancer progression.
As a member of the VICKZ family, IGF2BP1 is highly conserved across species and is expressed in a range of tissues during development. Its expression level has also been linked to certain diseases, such as neuroblastoma and breast cancer.
In conclusion, IGF2BP1 is a crucial mRNA-binding protein that plays a vital role in regulating gene expression and cellular processes. Its diverse functions make it an important target for further research in various fields, including cancer biology and neurodevelopmental disorders.

Source

Rabbit

Specificity

The antibody against IGF2BP1 was produced by immunizing rabbits with a synthetic peptide corresponding to amino acids 508-534 from the C-terminal region of the human IGF2BP1 protein. The peptide was conjugated to KLH for enhanced immunogenicity. To provide a distinct and diverse response, I will generate content based on the original information without following the conversational approach used by ChatGPT.

Species Reactivity

Human;Mouse

Predictive reaction species

X, M, R, C, Z

Application

FC~~1:25
IHC-P~~1:25
WB~~1:2000

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To keep the refrigerated item fresh and safe, keep it at a temperature between 2 and 8 degrees Celsius for a maximum of two weeks. If you want to store it for an extended period, it is advisable to freeze it at -20°C in small quantities to prevent the formation of ice crystals that can damage the product's quality. Avoid thawing and refreezing as it might affect the items' quality.

Storage buffer

The purified polyclonal antibody is provided in a phosphate-buffered saline (PBS) solution containing 0.09% (W/V) sodium azide as a preservative. The purification process involves passing the antibody through a protein A column to isolate it, followed by a peptide affinity purification step.

Research area

Others

Target

Background

IGF2BP1 is an RNA-binding factor that plays a critical role in mRNA transport and transient storage within cytoplasmic protein-RNA complexes, known as mRNPs. By recruiting target transcripts to these mRNPs, IGF2BP1 facilitates mRNA transport and storage while shielding them from endonuclease attacks or microRNA-mediated degradation. Additionally, IGF2BP1 modulates the rate and location at which target transcripts encounter the translational apparatus, playing a direct role in the transport and translation of transcripts required for axonal regeneration in adult sensory neurons.
One of the key functions of IGF2BP1 is its regulation of beta-actin/ACTB mRNA translation, which is crucial for cell polarity, cell migration, and neurite outgrowth. Co-transcriptionally associating with the ACTB mRNA in the nucleus, IGF2BP1 binds to a conserved 54-nucleotide element in the ACTB mRNA 3'-UTR, known as the 'zipcode', thus forming an RNP complex. This complex is then exported to the cytoplasm, where it binds to a motor protein and is transported along the cytoskeleton to the cell periphery. During transport, IGF2BP1 prevents ACTB mRNA from being translated into protein, and when the RNP complex reaches its destination near the plasma membrane, IGF2BP1 is phosphorylated, allowing ribosomal 40S and 60S subunits to assemble and initiate ACTB protein synthesis. Monomeric ACTB then assembles into the subcortical actin cytoskeleton.
IGF2BP1 also plays a crucial role in neuronal development, acting as a key regulator of neurite outgrowth, growth cone guidance, and neuronal cell migration, likely through spatiotemporal fine tuning of protein synthesis of genes such as ACTB. IGF2BP1 may also regulate mRNA transport to activated synapses and stabilizes transcripts such as ABCB1/MDR-1 mRNA, PTGS2 transcript, and BTRC/FBW1A mRNA.
Furthermore, IGF2BP1 binds to and stabilizes IGF2 mRNA, MYC mRNA, CD44 mRNA, H19 transcript, neuron-specific TAU mRNA, MAPK4 3'-UTR, and interacts with PTEN transcript open reading frame, preventing mRNA decay. By disrupting microRNA-dependent interaction with AGO2, IGF2BP1 prevents BTRC/FBW1A mRNA degradation, while promoting directed movement of tumor-derived cells by fine-tuning intracellular signaling networks. It also blocks the formation of infectious HIV-1 particles by interacting with HIV-1 GAG protein.
During cellular stress, such as oxidative stress or heat shock, IGF2BP1 stabilizes transcripts such as CD44, IGF2, MAPK4, MYC, PTEN, RAPGEF2, and RPS6KA5 that are recruited to stress granules. This collective action on multiple transcripts ensures their stability and prevents cell damage.

Celluar localization

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Cell projection, lamellipodium. Cell projection, dendrite. Cell projection, dendritic spine Cell projection, growth cone. Cell projection, filopodium. Cell projection, axon. Note=In the nucleus, located in discrete foci, coinciding with the sites of ACTB transcription (By similarity). In the cytoplasm, localizes in cytoplasmic mRNP granules. Colocalizes with microtubules in growth cone filopodia and along neurites in neuronal cells (By similarity). Cytoplasmic colocalization with ACTB mRNA is partially lost at the cell periphery, suggesting release of the transcript. In neuronal processes, exhibits fast retrograde and anterograde movements, when associated with ACTB mRNA; this motility is lost when the association is inhibited (By similarity). In hippocampal neurons, predominantly located within dendrites, particularly at dendritic branching points in young cells, compared to axons (By similarity). In axons, predominantly found in axonal branches and their growth cones (By similarity) In motile cells, such as migrating fibroblasts, localizes to leading edges where it colocalizes with microtubules and microfilaments and to retracting tails (By similarity). Dendritic levels are regulated by neuronal activity and glutaminergic signals: they are increased by KCl-induced depolarization, which induces rapid efflux from the cell body into dendrites, and decreased by the NMDA receptor agonist (By similarity). In motile cells, transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). In response to cellular stress, such as oxidative stress or heat shock, recruited to stress granules, but not to processing bodies.

UniPort

Q9NZI8

References

References

Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010)
Pillas, D., et al. PLoS Genet. 6 (2), E1000856 (2010) :
Noubissi, F.K., et al. Cancer Res. 69(22):8572-8578(2009)
Zhou, Y., et al. Virology 393(2):210-220(2009)
Kawakami, Y., et al. Biosci. Biotechnol. Biochem. 73(8):1811-1817(2009)