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Rabbit Anti-PMS2 Polyclonal Antibody#abs132770

Please note that the provided price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent. Western blot analysis of PMS2 expression in Hela cell lysate,The lane on the left is treated with the antigen-specific...

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Description

Catalog-specification	Delivery time	USD price
abs132770-50ug	1-2 Weeks	201.0
abs132770-100ug	1-2 Weeks	301.0

Please note that the provided price is only for your reference. For detailed pricing information, we kindly request you to get in touch with our sales representative, Vecent.

Overview
catalog	abs132770
Description	The post-replicative DNA mismatch repair system (MMR) consists of various components that work together to correct errors in DNA replication. One important component is MutL alpha, which forms a heterodimer with MLH1. MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) initially binds to a mismatched region in the DNA double helix, and then MutL alpha is recruited to this heteroduplex structure. The assembly of MutL-MutS-heteroduplex complex, along with the presence of RFC and PCNA, activates the endonuclease activity of PMS2. PMS2 introduces single-strand breaks near the mismatched region, creating new entry points for the exonuclease EXO1. EXO1 degrades the DNA strand that contains the mismatch, facilitating the removal of the erroneous nucleotides. To ensure that only the newly mutated DNA strand is corrected, DNA methylation plays a crucial role. Methylation prevents cleavage by the endonuclease, thereby ensuring that the repair machinery selectively operates on the strand with the mismatch. Additionally, MutL alpha (MLH1-PMS2) physically interacts with the clamp loader subunits of DNA polymerase III. This interaction suggests that MutL alpha may play a role in recruiting DNA polymerase III to the repair site, facilitating the synthesis of new DNA strands during the correction process. Furthermore, MutL alpha is implicated in DNA damage signaling, which is a cellular response to major DNA damage. This signaling process can induce cell cycle arrest and, in severe cases, trigger apoptosis to prevent the propagation of damaged DNA. In summary, the post-replicative DNA mismatch repair system involves the interaction of various components, including MutL alpha, to identify and correct errors in DNA replication. This intricate process ensures the maintenance of genomic integrity and the prevention of potentially harmful mutations.
Other names	The DNA mismatch repair gene homologue, known as PMS2, plays a critical role in repairing DNA mismatches. PMS2 is a protein that is essential for postmeiotic segregation, particularly in S. cerevisiae. The PMSL2 gene, also known as MLH4 or HNPCC4, produces the mismatch repair endonuclease PMS2. Postmeiotic segregation is increased when PMS2 is present, and this protein is a crucial component of the mismatch repair system. PMS1 homolog 2 is another name for PMS2, and it helps to ensure the integrity of genetic material. The PMS2CL protein is a variant form of PMS2 that may have a distinct role in DNA repair processes. Despite these various designations, PMS2 is a single, crucial component of the DNA mismatch repair system.
Source	Rabbit
Specificity	The endogenous levels of total PMS2 can be detected using the PMS2 antibody. This antibody specifically targets and recognizes the PMS2 protein, allowing for the detection and quantification of its total levels.
Species Reactivity	Human;Mouse
Antigen	PMS2
Application	The recommended dilution for Western blot is 1:500-1:2000, while for immunofluorescence and immunocytochemistry it is 1:100-1:500. For ELISA using a peptide, the suggested dilution range is 1:20000-1:40000.
Immunogen	A synthesized peptide.
MW	110 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;Plasma Membrane;
UniPort	P54278