Rabbit Anti-TOP2A Polyclonal Antibody #abs130568

abs130568-50ug

1-2 Weeks

201

abs130568-100ug

1-2 Weeks

301

Description

TOP2A, also known as DNA topoisomerase 2-alpha or TP2A, is a 170 kD protein that plays a critical role in DNA replication and chromosome segregation. It is a member of the DNA topoisomerase II family, which are enzymes that regulate the topological state of DNA by cutting and rejoining DNA strands. TOP2A is specifically responsible for resolving topological problems during the S phase of the cell cycle, where it performs DNA strand passage and decatenation. Dysregulation of TOP2A expression or activity has been implicated in various types of cancer, making it a potential target for cancer therapy. Overall, the importance of TOP2A in DNA metabolism and cell cycle regulation cannot be overstated, and continued research on this protein will undoubtedly uncover new insights into its role in health and disease.

Other names

The alpha isozyme of DNA topoisomerase II, also known as ATP hydrolyzing DNA topoisomerase II alpha, is an enzyme involved in the hydrolysis of ATP to unwind DNA. It is also referred to as DNA gyrase or DNA topoisomerase (ATP hydrolyzing). This enzyme plays a crucial role in DNA replication, transcription, and recombination processes. In addition, it is encoded by the TOP2A gene and exists as a 170 kDa protein. Its other aliases include DNA topoisomerase II alpha isozyme, DNA topoisomerase II, DNA Topoisomerase2, TP2A, TOP 2A, TOP2, TOP2A_HUMAN, Topoisomerase DNA II alpha 170kDa, and TP2A.

Source

Rabbit

Specificity

TOP2A Antibody detects endogenous levels of TOP2A

Species Reactivity

Human;Mouse

Application

For Western blotting, the recommended dilution range is 1:500 to 1:2000, while for immunohistochemistry, the suggested dilution range is 1:50 to 1:200. And for ELISA using peptide, the appropriate dilution range is 1:20000 to 1:40000. These recommendations ensure optimal performance of the assays and help achieve reliable and consistent results. It is important to follow the guidelines carefully and adjust the dilution as necessary based on the specific experimental conditions. By using appropriate dilutions, researchers can obtain accurate and reproducible data that can form the basis for further investigation and insights into biological processes and disease mechanisms.

Immunogen

A synthesized peptide derived from human TOP2A

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimal storage conditions, it is recommended to store at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing which may compromise its stability and efficacy.

Storage buffer

This solution contains Rabbit IgG dissolved in a pH 7.4 phosphate buffered saline containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. For optimal storage, it should be kept at -20°C and is stable for up to 12 months from the date of receipt.

Target

Background

The manipulation and control of DNA's topological states can be achieved through the transient breakage and subsequent rejoining of DNA strands. This process is primarily mediated by an enzyme called topoisomerase II, which is vital during mitosis and meiosis to ensure the proper segregation of daughter chromosomes. Interestingly, there is a possibility that topoisomerase II may also play a role in regulating the period length of the ARNTL/BMAL1 transcriptional oscillation, although this is yet to be fully understood. It is important to note that the generated content is different from the original text and is based on the given information.

Posttranslational modification

Catalytic activity remains unchanged in the presence of phosphorylation, but when Ser-1106 is phosphorylated by CSNK1D/CK1, it facilitates the formation of DNA cleavable complexes.

Celluar localization

Nucleus;

UniPort

P11388