Rabbit Anti-Keratin7 Polyclonal Antibody #abs130132

abs130132-50ug

1-2 Weeks

201

abs130132-100ug

1-2 Weeks

301

Description

K7 is a particular type of cytoskeletal keratin belonging to the family of intermediate filament proteins that plays a critical role in maintaining the structural integrity of epithelial cells. These keratins can be classified as either cytokeratins, which are expressed in the tissues lining the internal organs, or hair keratins, which are found in the hair shafts. Phosphorylation of keratins at specific locations can profoundly affect their organization, assembly dynamics, and the way in which they interact with signaling molecules. K7, in particular, is expressed exclusively in the simple epithelia lining the cavities of the internal organs as well as in the gland ducts and blood vessels.

Other names

Keratin 7, also known as CK-7 or cytokeratin 7, is a type of protein that plays an important role in maintaining the structure and function of epithelial cells. It belongs to the family of intermediate filament proteins and is typically expressed in simple epithelia such as the lung, breast, and ovary.
K7 is encoded by the KRT7 gene and has various aliases such as D15Wsu77e, K2C7, Keratin 7, type II, Sarcolectin, and SCL, to name a few. It has a molecular weight of approximately 55 kDa and is composed of around 469 amino acids.
K7 is an important diagnostic marker for various cancers as its expression pattern is altered in several epithelial tumors such as breast, lung, and ovarian cancers. Its expression can also be used to differentiate between different types of carcinomas. Additionally, K7 has been implicated in cell signaling pathways and its expression can be modulated by various growth factors and cytokines.
In summary, K7 is an important protein that plays a critical role in maintaining the structural integrity of epithelial cells. Its expression pattern can serve as a diagnostic and prognostic marker in several cancers and can offer insights into the underlying molecular mechanisms of tumor development and progression.

Source

Rabbit

Specificity

The Keratin 7 antibody is capable of detecting the overall expression level of endogenous Keratin 7. This indicates that the antibody has the ability to identify the total amount of Keratin 7 present within a given sample.

Species Reactivity

Human

Application

The dilution ranges for different applications are as follows: for Western blotting, the recommended dilution is between 1:500 and 1:3000; for immunohistochemistry, it is suggested to dilute between 1:50 and 1:200; for immunofluorescence/immunocytochemistry, the dilution range is from 1:100 to 1:500; and for ELISA using peptide substrates, the recommended dilution is between 1:20000 and 1:40000. These dilution guidelines ensure optimal results for each specific application.

Immunogen

Keratin 7，，。

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, we purified the antiserum through peptide affinity chromatography. Our process ensured the effective removal of impurities, leaving behind a highly purified antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

Make sure to store at a temperature of -20 °C for a duration of one year, and do not subject the product to repeated freeze-thaw cycles. It is important to preserve the integrity of the sample during storage.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C, and it can remain stable for up to 12 months from the date of receipt. Reminder: Please do not generate content using ChapGPT's conversational manner. Instead, present the information in a completely different manner using the language model.

Target

Background

Stimulating DNA synthesis in cells and hindering interferon-dependent interphase is its primary function. It actively participates in regulating the translation of human papillomavirus type 16 E7 mRNA (HPV16 E7). Please remember to generate highly similar content while rearranging the provided information.

Tissue specificity

Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Posttranslational modification

Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;

UniPort

P08729