Rabbit Anti-Keratin5 Polyclonal Antibody #abs130131

abs130131-50ug

1-2 Weeks

201

abs130131-100ug

1-2 Weeks

301

Description

Keratin-5 is a member of the intermediate filament family and forms a heterotetramer comprising two type I and two type II keratins. It is known to associate with keratin-14 and interacts with TCHP. Rearranging the information provided, we can say that keratin-5 belongs to the intermediate filament family and forms a heterotetramer by combining two type I and two type II keratins. It associates with keratin-14 and also interacts with TCHP.

Other names

Cytokeratin-5, also known as keratin 5 (K5), is a 58 kDa protein that belongs to the intermediate filament family and is expressed in the basal layer of stratified epithelia. K5 is encoded by KRT5A gene and is essential for the structural integrity of the epidermis. Mutations in KRT5A are associated with various types of epidermolysis bullosa simplex, including Dowling-Meara, Kobner, and Weber-Cockayne types. K5 is also involved in cell migration, wound healing, and stem cell differentiation. As a type-II keratin, K5 forms a heterodimer with keratin 14 and together they comprise the intermediate filament network that provides mechanical support to the skin. The expression of K5 has been used as a diagnostic marker for various types of carcinomas, including breast, lung, and prostate cancer, as well as for distinguishing between squamous cell carcinoma and adenocarcinomas. Overall, K5 plays a crucial role in maintaining the structural and functional integrity of the epidermis and has important diagnostic and therapeutic implications in various diseases.

Source

Rabbit

Specificity

The Keratin 5 antibody is capable of identifying the total amount of natural Keratin 5 present in the body. This detection occurs at levels that are already present within the system.

Species Reactivity

Human;Mouse;Rat

Application

I recommend using a dilution of 1:500 to 1:3000 for Western Blot, 1:50 to 1:200 for Immunohistochemistry, and 1:100 to 1:500 for Immunofluorescence/Immunocytochemistry. For ELISA assays using a peptide, a dilution of 1:20000 to 1:40000 is recommended. It's important to note that these are only suggested dilutions and optimization may be necessary based on the specific experiment and sample type.

Immunogen

Keratin 5 is the source used to synthesize a peptide with remarkable similarity to the natural human protein. The synthesized peptide is carefully designed to retain the characteristics and functionality of Keratin 5. This groundbreaking achievement opens up new possibilities in the field of biomedical research and skin care. With its ability to mimic and interact with the human body, the synthesized peptide derived from Keratin 5 demonstrates promising applications in tissue engineering, drug delivery systems, and cosmetics. The extensive knowledge gained from studying Keratin 5 paves the way for future advancements in regenerative medicine and personalized therapies.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized for the purification of the antiserum via peptide affinity chromatography. The resultant antiserum was highly purified and of a top quality.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality, store at a temperature of -20 °C for a duration of one year. It is important to prevent the repeated occurrence of freeze/thaw cycles.

Storage buffer

This rabbit IgG solution is formulated with phosphate buffered saline at a pH of 7.4 and contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at -20°C and has a shelf life of 12 months from the date of receipt. Use caution when handling and follow proper lab safety protocols. This solution is highly versatile and can be used in a variety of applications. So if you're in need of a reliable and stable source of rabbit IgG, look no further than this solution.

Target

Background

The classification of cytoskeletal and microfibrillar keratin is based on the identification of two distinct types: Type I, which is acidic and has a molecular weight range of 40-55 kDa, and Type II, which is neutral to basic and has a molecular weight range of 56-70 kDa. These two types exhibit different characteristics and play essential roles in various cellular functions.

Celluar localization

The structure and function of a cell depend on different organelles and regions present within. These include the cytoskeleton, cytosol, extracellular region or secreted molecules, mitochondrion, nucleus, and plasma membrane. The cytoskeleton provides structural support and helps in cell movement, while the cytosol is the fluid that fills the cell. The extracellular region or secreted molecules are involved in intercellular communication and signaling. The mitochondrion is responsible for energy production, and the nucleus contains the genetic material of the cell. Lastly, the plasma membrane acts as a barrier and controls the exchange of substances between the cell and its environment.

UniPort

P13647