Rabbit Anti-Keratin8 Polyclonal Antibody #abs130133

abs130133-50ug

1-2 Weeks

201

abs130133-100ug

1-2 Weeks

301

Description

K8 is a type II cytoskeletal keratin, which plays a vital role in maintaining the structural integrity of epithelial cells. The keratin family of intermediate filament proteins is further divided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites can impact their organization, assembly dynamics, and their interaction with signaling molecules. The p38 kinase is responsible for phosphorylating K8, which regulates cellular keratin filament reorganization. This process is crucial for maintaining the stability and function of epithelial tissues in the body. Overall, K8 is a critical component of the cytoskeleton, and it plays an essential role in preserving the structural integrity of epithelial cells.

Other names

Keratin type II cytoskeletal 8, also known as cytokeratin-8 (CK8), is a protein that plays a crucial role in the structure and function of epithelial tissues. It is encoded by the KRT8 gene and belongs to the family of intermediate filaments. CK8 is expressed in a wide range of tissues, including the liver, pancreas, lung, and kidney.
This protein is involved in maintaining the integrity of epithelial cells by providing mechanical strength and resistance to stress. It also plays a role in cell signaling and gene regulation, as well as in the process of apoptosis (programmed cell death).
Mutations in the KRT8 gene have been linked to various diseases, including liver and pancreatic disorders. Additionally, CK8 is used as a biomarker in cancer diagnosis and prognosis, as it is overexpressed in many types of cancer, including breast, ovarian, and lung cancers.
Overall, CK8 is a crucial protein that contributes to the function and maintenance of various tissues in the body. Its importance in disease diagnosis and treatment makes it a valuable target for further research and development of therapeutics.

Source

Rabbit

Specificity

The Keratin 8 antibody is capable of identifying the presence of total Keratin 8 within the body's natural levels.

Species Reactivity

Human;Mouse;Rat

Application

ELISA (peptide) 1:20000-1:40000, WB 1:500~1:3000, IHC 1:50~1:200, and IF/ICC 1:100-1:500 are the recommended dilutions for different applications. These dilutions are based on the original text information provided.

Immunogen

，8。，ChapGPT，。

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure quality preservation, it is recommended to keep this product at a temperature of -20 °C for up to one year. It is important to avoid exposing it to repetitive cycles of freezing and thawing to maintain its effectiveness.

Storage buffer

This rabbit IgG is preserved in a solution of phosphate buffered saline with a pH level of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended that you keep it securely stored at a temperature of -20°C to maintain its integrity. It remains stable for a year from the date of receipt.

Target

Background

Linking the contractile apparatus to dystrophin at the costameres of striated muscle is facilitated by KRT19. Let's rearrange the provided content to generate highly similar content: KRT19 plays a crucial role in connecting the contractile apparatus to dystrophin at the costameres found in striated muscle.

Tissue specificity

Dystrophin and spectrin are present in structures located at the sarcolemma membrane, specifically in the costameres of myoplasm. These structures accumulate in muscle fibers and have been observed in various studies. Interestingly, the expression of these same proteins has also been identified in the hard palate and gingival mucosa of the oral cavity. Overall, these findings suggest that dystrophin and spectrin play important roles in maintaining the integrity of cell membranes in both muscle and non-muscle tissues.

Posttranslational modification

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

UniPort

P05787