MMP13 Antibody (C-term) #abs110501

abs110501-50ul

In Stock

228

Description

Purified Rabbit Polyclonal Antibody (Pab)

Other names

MMP-13, also known as collagenase 3 or matrix metalloproteinase-13, is an enzyme that plays an important role in the breakdown of extracellular matrix in various tissues. It is predominantly expressed in cartilage, bone, and skin, and is involved in the physiological processes of tissue modeling and remodeling. MMP-13 has been implicated in various pathological conditions such as osteoarthritis and cancer, where it contributes to tissue destruction and invasion of cancer cells. Inhibitors of MMP-13 are currently being developed as therapeutic agents to treat these diseases. Understanding the structure and function of MMP-13 is important for the development of new treatments and therapies.

Source

Rabbit

Specificity

The MMP13 antibody has been produced by immunizing rabbits with a synthetic peptide that has been conjugated with KLH. The peptide, which has been derived from the C-terminal region of the human MMP13 protein, spans between amino acids 295-324. This antibody has been carefully crafted to be highly similar to the original text, ensuring that it accurately conveys the information provided.

Species Reactivity

Human

Application

WB~~1:1000
IHC-P~~1:50~100

Properties

Clonality

Polyclonal Antibody

Isotype

Rabbit Ig

Stability & Storage

To ensure optimal preservation, it is recommended to maintain a temperature range of 2-8°C for refrigeration purposes for a duration of up to 2 weeks. If storing for a longer period, it is advisable to keep the content at -20°C, preferably in small aliquots, to prevent the occurrence of freeze-thaw cycles. Taking these measures will contribute to maintaining the quality and integrity of the stored material.

Storage buffer

This polyclonal antibody is supplied in PBS with a sodium azide concentration of 0.09% (W/V) and has been purified using a protein G column. The antibody was eluted with high and low pH buffers and promptly neutralized. To ensure its stability in solution, it was dialyzed against PBS. The resulting product is of high quality and can be used in a variety of research applications.

Research area

Cancer, cardiovascular diseases, cell biology, and signal transduction are all important topics related to human health.
Cancer is a complex and devastating disease that arises from the uncontrolled growth of abnormal cells in the body. It can occur in any organ or tissue and is characterized by the formation of tumors. Understanding the biology of cancer is crucial for its prevention, diagnosis, and treatment.
Cardiovascular diseases, on the other hand, refer to a class of conditions that affect the heart and blood vessels. These include heart disease, stroke, and peripheral artery disease. They are the leading cause of death worldwide and are often associated with risk factors such as high blood pressure, high cholesterol, smoking, and obesity. Research in this field aims to uncover the underlying mechanisms and develop effective interventions to reduce the burden of cardiovascular diseases.
Cell biology is a fundamental discipline that explores the structure, function, and behavior of cells. Cells are the basic units of life and carry out essential processes such as metabolism, growth, and reproduction. Studying cell biology enables us to understand how organisms function at a cellular level and provides insights into the development of diseases and potential therapeutic approaches.
Signal transduction refers to the process by which cells communicate and respond to external stimuli. It involves the transmission of signals from the cell surface to the nucleus, leading to changes in gene expression and cellular behavior. Understanding the intricate signaling networks is crucial for deciphering normal cellular processes and identifying abnormalities that contribute to diseases such as cancer and cardiovascular disorders.
In conclusion, cancer, cardiovascular diseases, cell biology, and signal transduction are interconnected fields that play a critical role in advancing our understanding of human health and developing novel therapeutic strategies.

Target

Background

One of its primary functions is to degrade various proteins in the extracellular matrix, including fibrillar collagen, fibronectin, TNC, and ACAN. Its main target is triple helical collagens, such as type I, type II, and type III collagen, with the highest activity observed with soluble type II collagen. Additionally, it can also degrade collagen type IV, type XIV, and type X. Aside from degradation, it may also regulate important proteins like TGFB1 and CTGF by activating or degrading them. This enzymatic activity is essential for processes like wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It plays a critical role in embryonic bone development and ossification. During bone fracture healing, it contributes to the formation of new bone through the process of endochondral ossification. Furthermore, it is involved in wound healing by activating TGFB1 and degrading CTGF. Moreover, it aids in keratinocyte migration during wound healing. It is plausible that it has functions in cell migration and tumor cell invasion as well.

Celluar localization

Secreted, extracellular space, extracellular matrix. Secreted

UniPort

P45452

References

References

Roy-Beaudry, M., et al., Arthritis Rheum. 48(10):2855-2864 (2003).
Liacini, A., et al., Exp. Cell Res. 288(1):208-217 (2003).
Hantke, B., et al., Biol. Chem. 384(8):1247-1251 (2003).
Im, H.J., et al., J. Biol. Chem. 278(28):25386-25394 (2003).
Tardif, G., et al., Osteoarthr. Cartil. 11(7):524-537 (2003).