Rabbit Anti-VEGF Polyclonal Antibody #abs123646

abs123646-50ul

In Stock

201

abs123646-100ul

In Stock

301

Other names

VEGF A, also known as vascular endothelial growth factor A or VEGFA, is a key regulator of angiogenesis, which is the formation of new blood vessels from existing ones. It plays a crucial role in various physiological and pathological processes, including embryonic development, wound healing, and tumor progression.
The isoform 2 precursor of VEGF A is an alternative form of the protein that contains additional amino acids. This isoform has been found to have distinct biological functions compared to the standard isoform.
VEGF, also referred to as Vegf, is a widely used abbreviation for vascular endothelial growth factor. It is an important signaling protein involved in stimulating the formation of blood vessels.
MGC70609 and MVCD1 are alternative names or aliases for VEGF A, which are often used in scientific literature or databases.
VEGFA_HUMAN is a shorthand notation used to denote the human version of VEGF A, distinguishing it from other species variations.
Vascular permeability factor (VPF) is an older term originally used to describe VEGF A due to its ability to increase the permeability of blood vessels.
In summary, VEGF A, or Vascular endothelial growth factor A, is a critical protein involved in angiogenesis and has various isoforms and aliases. It plays a crucial role in numerous biological processes and is essential for normal development and disease progression.

Source

Rabbit

Specificity

VEGF

Species Reactivity

Human;Dog;Pig;Cow;Horse;Rabbit;Sheep;

Application

The binding affinity of a specific antibody can be determined by various methods, including Western blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry on Paraffin-Embedded Sections (IHC-P), Immunohistochemistry on Frozen Sections (IHC-F), and Immunofluorescence (IF). The recommended dilutions for these techniques are as follows:
- WB: The antibody is typically used at a dilution of 1:500-2000.
- ELISA: A dilution of 1:500-1000 is commonly used in ELISA experiments.
- IHC-P: For immunohistochemical staining on paraffin-embedded sections, the antibody is recommended to be diluted at 1:400-800.
- IHC-F: Immunohistochemistry on frozen sections can be performed using the antibody at a dilution of 1:400-800.
- IF: For immunofluorescence experiments, the antibody is typically used at a dilution of 1:100-500.
It is important to note that when performing IHC on paraffin sections, antigen retrieval is necessary to ensure accurate results. Overall, these dilution guidelines can serve as a starting point for optimizing antibody concentrations in various experimental setups.

Immunogen

A synthetic peptide sourced from the human VEGF molecule at position 81-132/232 has been conjugated with KLH. With this conjugation process, highly similar content is produced that draws on the original text's information.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

The product should be stored at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated freeze/thaw cycles during storage.

Storage buffer

A solution containing 0.01M TBS at pH 7.4 has been developed, which includes 1% BSA, 0.03% Proclin 300, and 50% glycerol. This formulation provides an optimal environment for a variety of biological applications. The addition of BSA ensures that proteins are stabilized and protected from denaturation, while Proclin 300 serves as a bacteriostatic agent to prevent bacterial contamination. The presence of glycerol allows for improved sample storage, reducing the potential for freezing damage or other forms of degradation. Overall, this solution provides a reliable and effective tool for researchers in a variety of fields.

Research area

Cell biology is a fundamental aspect of understanding tumor growth and development in the cardiovascular system. Immunology plays a crucial role in regulating the body's response to tumor cells and their proliferation. Signal transduction pathways are essential for transmitting signals within cells, and they play a significant role in tumor development. Growth factors and hormones contribute to the growth and survival of tumor cells by stimulating their division and inhibiting cell death. Vascular endothelial cells are important for the formation of new blood vessels, which supply nutrients and oxygen to tumor cells, aiding in their growth and metastasis. Overall, the intricate interplay between these factors contributes to the complex nature of tumor biology in the cardiovascular system.

Target

Background

This particular protein, a member of the PDGF/VEGF growth factor family, plays a crucial role in various biological processes. Its primary function is to stimulate the growth and development of endothelial cells. By doing so, it influences several effects, such as enhancing vascular permeability, initiating angiogenesis and vasculogenesis, promoting the growth of endothelial cells, facilitating cell movement, and preventing cell death. The gene responsible for encoding this protein has multiple alternative forms, resulting in different variations of the protein. Some transcript variants also exhibit alternative translation initiation sites, leading to the production of additional isoforms. These findings, as provided by RefSeq, highlight the diverse functionality and complexity of this gene product.

Celluar localization

Extracellular matrix secreted protein

UniPort

P15692