Rabbit Anti-FOX3/NeuN Polyclonal Antibody #abs123626

abs123626-50ul

In Stock

201

abs123626-100ul

In Stock

301

Other names

NeuN, also known as FOX3, is a crucial neuronal marker that plays a significant role in identifying and characterizing neuronal nuclei. It is a Vertebrate neuron-specific nuclear protein, also referred to as neuronal nuclear antigen A60 or Neuna60. Additionally, it is an isoform 1 of the RNA binding protein fox-1 homolog 3, which is essential for various cellular processes. Moreover, it is associated with the RNA binding protein, fox 1 homolog (C. elegans) 3 or Rbfox3. Other aliases for NeuN include D11Bwg0517e, Fox-3, Hrnbp3, RP23-159O6.5, and FLJ56884. It is worth mentioning that NeuN is a novel protein that is categorized as the Fox-1 homolog C or fox1 homolog C. Furthermore, it is associated with FLJ58356 and hexaribonucleotide binding protein 3 RFOX3_HUMAN. Overall, NeuN is a critical component in the study and characterization of neuronal nuclei.

Source

Rabbit

Specificity

NeuN

Species Reactivity

Human;Mouse;Rat;Dog;Cow;Horse;

Application

To detect a certain antigen, the ELISA method requires a dilution of 1:500-1000, while the IHC-P and IHC-F methods require a dilution of 1:400-800. For IF, the recommended dilution is 1:100-500, but if a paraffin section is used, antigen retrieval is necessary. It is important to note these dilution recommendations to ensure accurate results.

Immunogen

A synthetic peptide derived from human NeuN (75-118aa) has been conjugated with KLH. This peptide corresponds to amino acid residues 51-150 out of a total of 312 residues. To produce similar content, the information can be rearranged as follows: KLH has been conjugated with a synthetic peptide derived from human NeuN (75-118aa), which corresponds to amino acid residues 51-150 out of a total of 312 residues.

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To preserve the compound's integrity, it is recommended to store it at -20 °C for up to one year. It is crucial to avoid repetitive freeze/thaw cycles that may damage the material.

Storage buffer

A solution containing 1% BSA, 0.03% Proclin300 and 50% Glycerol in 0.01M TBS at pH7.4 has been prepared. This solution is ideal for various research applications and provides a stable environment for biological samples. The addition of BSA ensures that there is minimal sample loss while the presence of Proclin300 helps to prevent bacterial growth. The high glycerol content helps to maintain the stability of the samples and prevents degradation. Overall, this solution is an excellent choice for researchers who require a stable and reliable solution for their experiments.

Research area

Cell Biology Neurobiology Transporter Epigenetics

Target

Background

NeuN, also known as Neuronal Nuclei, is a specialized nuclear protein that serves as a reliable marker for neurons in various biological settings. Its effectiveness has been demonstrated in primary cultures and retinoic acid-stimulated P19 cells, as well as in identifying neurons in transplantation experiments. In vertebrates, NeuN is specifically found in neurons and exhibits DNA-binding properties.
In the case of mice, NeuN is present in different types of neuronal cells found throughout the nervous system, including the cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord, dorsal root ganglia, sympathetic chain ganglia, and enteric ganglia of the peripheral nervous system. Notably, the expression of NeuN is only observed in neurons that have ceased dividing and are undergoing cellular and morphological differentiation. It is absent in regions characterized by cell proliferation.
This unique protein has proven to be a valuable immunohistochemical marker for studying excitotoxic brain lesions and diagnosing various human tissue samples from both the central and peripheral nervous systems. By detecting the presence of NeuN through immunohistochemistry, researchers and clinicians gain insights into neural development, neural damage, and various neurological disorders.

Celluar localization

Nucleus cytoplasm

UniPort

A6NFN3