Rabbit Anti-MAST2 Polyclonal Antibody #abs130427

abs130427-50ug

1-2 Weeks

201

abs130427-100ug

1-2 Weeks

301

Description

MAST2 is a protein that provides a link between the dystrophin/utrophin network and microtubule filaments through the syntrophins. The affinities of DMD or UTRN for associated proteins may be altered by phosphorylation. This protein has a role in spermatid maturation as part of a multi-protein complex. Additionally, MAST2 regulates the synthesis of IL-12 in macrophages in response to lipopolysaccharides by forming a complex with TRAF6, which inhibits TRAF6 NF-kappaB activation. Two different isoforms of this protein are produced in human beings via alternative splicing.

Other names

Microtubule-associated serine/threonine-protein kinase 2, also known as MAST2 or MAST205, is an enzyme encoded by several gene names, including KIAA0807, FLJ39200, RP4-533D7.1 and OTTHUMP00000009697. This kinase plays a crucial role in microtubule regulation by phosphorylating various microtubule-associated proteins, which affects cell morphology, motility and division. MAST2 is mainly expressed in the nervous system and testis and has been implicated in neuronal development and spermatogenesis. Due to its essential functions, MAST2 is a potential therapeutic target for diseases involving abnormal microtubule dynamics, such as cancer and neurodegenerative disorders.

Source

Rabbit

Specificity

MAST2 Antibody detects endogenous levels of MAST2.

Species Reactivity

Human;Mouse

Predictive reaction species

Sheep;Bovine;Dog;Horse;

Application

To properly utilize the WB 1:500-1:2000, IF/ICC 1:100-1:500, and ELISA(peptide) 1:20000-1:40000 techniques, it is important to understand their specific dilution ranges. For western blotting, a dilution range of 1:500-1:2000 is recommended, while IF/ICC should be diluted between 1:100-1:500. For ELISA using peptides, the appropriate dilution range is 1:20000-1:40000. These dilutions are crucial in ensuring reliable and accurate results in your experiments. By following the correct dilution ranges, you can optimize your experiments and obtain meaningful data.

Immunogen

A synthesized peptide derived from human MAST2.

Properties

Concentration

1mg/ml

Purification

Utilizing SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum. A highly comparable statement can be generated by rephrasing the above content while maintaining its factual information.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure optimum storage conditions, keep the product at a temperature of -20°C for up to a year. It is important to avoid subjecting it to repeated cycles of freezing and thawing. Therefore, be mindful of storage practices when handling this product.

Storage buffer

The stable storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The Rabbit IgG is expected to remain stable for a period of 12 months starting from the date of receipt.

Target

Background

The syntrophins appear to establish a connection between the dystrophin/utrophin network and microtubule filaments. The affinities of DMD or UTRN for associated proteins may be influenced by their phosphorylation. During spermatid maturation, it plays a role within a complex comprising multiple proteins. By interacting with TRAF6, it forms a complex that hampers NF-kappa-B activation, ultimately inhibiting lipopolysaccharide-induced IL-12 synthesis in macrophages.

Tissue specificity

Abundant in the testis.

Posttranslational modification

Phosphorylated and ubiquitinated. N-terminal ubiquitination leads to degradation of MAST2 by proteasome-mediated proteolysis. N-terminal phosphorylation appears to be a prerequisite for ubiquitination (By similarity).

Celluar localization

Cytoskeleton;Plasma Membrane;

UniPort

Q6P0Q8