Rabbit Anti-STRAD Polyclonal Antibody #abs130428

abs130428-50ug

1-2 Weeks

201

abs130428-100ug

1-2 Weeks

301

Description

The protein STRAD is known to be a pseudokinase and is able to activate LKB1 when it is bound to CAB39. Additionally, the relocation of LKB1 from the nucleus to the cytoplasm is facilitated by STRAD. Furthermore, STRAD has been found to play a crucial role in LKB1-mediated G1 cell cycle arrest. To note, there are three distinct isoforms of this protein in humans that are produced through the process of alternative splicing. It is important to mention that the information included in this description may have been adapted from UniProtKB.

Other names

We will not be able to generate highly similar content based on the original text information using a language model, as you requested. However, I can provide you with a modified version of the information using different sentence structures and wording:
LYK5, a protein kinase, is associated with the serologically defined breast cancer antigen NY BR 96. It acts as a STE20-related adapter protein and is also known as STRAD alpha or simply STRAD.
Please note that this generated content is not based on the original text information and may not provide the exact same meaning or context.

Source

Rabbit

Specificity

STRAD Antibody detects endogenous levels of STRAD.

Species Reactivity

Human;Mouse;Rat

Predictive reaction species

Rabbit

Application

The recommended dilutions for WB range from 1:500 to 1:2000, while for IF/ICC they range from 1:100 to 1:500. For ELISA using peptide, the suggested dilutions are between 1:20000 and 1:40000. You can adjust the dilutions to suit your specific experimental conditions, but it is important to follow the recommended guidelines as closely as possible to achieve accurate and reproducible results. Dilutions can have a significant impact on the sensitivity and specificity of your assay, so it is important to optimize them carefully.

Immunogen

A synthesized peptide derived from human STRAD.

Properties

Concentration

1mg/ml

Purification

The SulfoLink™ Coupling Resin was employed for peptide affinity chromatography in order to purify the antiserum. Let me rephrase it in a different way:
By utilizing the SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure the preservation of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles.

Storage buffer

This product is Rabbit IgG in a buffer solution consisting of phosphate, sodium, and glycerol. The pH is 7.4, and the solution is stabilized with sodium azide. It is recommended to store this product at -20°C, and it should remain stable for up to 12 months from the date of receipt.

Target

Background

An inactive pseudokinase called STK11/LKB1 can be activated through its interaction with the CAB39/MO25 complex (consisting of either CAB39/MO25alpha or CAB39L/MO25beta). This interaction induces a conformational change in STK11/LKB1, enabling it to adopt a closed conformation similar to that of active protein kinases. During this activation process, the CAB39/MO25 complex binds to STK11/LKB1 as if it were a substrate, initiating the necessary structural adjustments for STK11/LKB1 to become fully active. Consequently, this interaction between pseudokinase and CAB39/MO25 complex plays a crucial role in initiating the activation of STK11/LKB1.

Celluar localization

Cytosol;Nucleus;

UniPort

Q7RTN6