Rabbit Anti-Collagen XIV Alpha1 Polyclonal Antibody #abs130429

abs130429-50ug

1-2 Weeks

201

abs130429-100ug

1-2 Weeks

301

Description

The role of COL14A1 is to function as an adhesive component that helps integrate collagen bundles within the body. It is likely associated with the outer layer of interstitial collagen fibrils through its interaction with COL1. The COL2 domain of COL14A1 acts as a sturdy arm, extending out from the fibril and projecting the large N-terminal globular domain into the extracellular space. This enables potential interactions with other molecules present in the matrix or receptors on the surface of cells. COL14A1 belongs to the family of fibril-associated collagens with interrupted helices (FACIT). To sum up, COL14A1 plays a critical role in connecting collagen bundles, and its structure allows it to interact with other molecules in the extracellular space.

Other names

Alpha 1(XIV) chain of collagen, also known as undulin, is a protein that belongs to the collagen type XIV alpha 1 family. It is closely related to fibronectin and tenascin. Undulin, also referred to as collagen type XIV alpha 1, plays a significant role in the structure and function of connective tissues in the human body. Its involvement in various biological processes makes it an essential molecule for maintaining tissue integrity. Undulin, commonly known as collagen alpha 1(XIV) chain, is a protein with diverse functions that contribute to the overall health and well-being of an individual.

Source

Rabbit

Specificity

The Collagen XIV alpha 1 Antibody has the ability to recognize the presence of Collagen XIV alpha 1 at its natural levels in the body. The detection is based on the endogenous levels of this protein, making it an accurate and reliable tool in various research applications.

Species Reactivity

Human;Mouse

Predictive reaction species

Rabbit;Xenopus;Dog;Bovine;

Application

The recommended dilutions for using this antibody in different applications are as follows: For IHC, a dilution of 1:50 to 1:200 is recommended. For IF/ICC, a dilution of 1:100 to 1:500 is recommended. For ELISA with peptide, a dilution of 1:20,000 to 1:40,000 is recommended. Please note that the exact dilution may need to be optimized for individual experiments, based on the specific application and sample type. Regardless of the dilution used, it is important to ensure proper controls are in place to validate the antibody's specificity and reliability.

Immunogen

One possible rearranged version:
An alpha 1 peptide synthesized from human Collagen XIV has been developed, based on the sequence of this protein. This novel peptide is highly similar to the natural collagen and has potential applications in various fields. Collagen XIV is an important component of the extracellular matrix, where it participates in cell adhesion, tissue development, and maintenance. The alpha 1 chain of this protein contains several domains that are involved in binding to other proteins, such as integrins and proteoglycans. By mimicking these domains, the synthetic peptide can interact with the same partners and exert similar effects. Moreover, this peptide can be modified or conjugated with other molecules to enhance its properties or target specific tissues. The use of synthetic peptides derived from collagen is a promising approach to develop new biomaterials, drugs, or diagnostic tools.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed to perform peptide affinity chromatography for purification of the antiserum. Let's rearrange the content while preserving the original information.

Clonality

Polyclonal Antibody

Stability & Storage

To ensure storage longevity, keep the product at a temperature of -20 °C for a period of one year. It is important to avoid any sort of repeated freeze/thaw cycles as it can affect the quality of the product. Please adhere to these instructions for the best storage outcome.

Storage buffer

This product is Rabbit IgG that comes in phosphate buffered saline with a pH level of 7.4. It is also mixed with 150mM of NaCl, 0.02% sodium azide, and 50% glycerol, and should be stored at a temperature of -20°C. This product has a shelf-life of 12 months from the date of receipt, making it an ideal choice for long-term storage. Invest in this product today and have peace of mind knowing that you have a reliable solution for your research needs.

Target

Background

The primary function of this component is to act as an adhesive agent, facilitating the merging of collagen bundles. It is likely that this component is linked to the surface of interstitial collagen fibrils through its association with COL1. Once attached, the COL2 domain extends as a stiff arm from the fibril, projecting the large N-terminal globular domain into the extracellular space. In this region, it is possible for the globular domain to interact with other molecules present in the extracellular matrix or receptors on the surface of cells. The importance of this interaction should not be underestimated as it plays a crucial role in maintaining structural integrity and facilitating communication within the cellular environment. Overall, this component acts as a vital mediator, promoting cohesion and interaction within the extracellular matrix.

Posttranslational modification

Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.May contain numerous cysteine residues involved in inter- and intramolecular disulfide bonding.

Celluar localization

Endoplasmic reticulum;Extracellular region or secreted;

UniPort

Q05707