Rabbit Anti-Maf Polyclonal Antibody #abs130382

abs130382-50ug

1-2 Weeks

201

abs130382-100ug

1-2 Weeks

301

Description

Maf is a transcriptional regulator that can act as an activator or repressor. Its main role is in the development of embryonic lens fiber cells. During this process, Maf recruits coactivators such as CREBBP and EP300 to activate the expression of crystallin genes, which are crucial for lens fiber cell differentiation.
In addition to lens development, Maf also plays a role in other cellular processes. It activates the expression of IL4 in T helper 2 (Th2) cells, which is important for immune responses. Maf can also increase the susceptibility of T-cells to apoptosis by interacting with MYB and decreasing the expression of BCL2, a protein that inhibits cell death.
Maf is known to form a complex with PAX6 to strongly activate the glucagon gene promoter through a specific element called G1. This complex is involved in regulating glucagon expression, which is crucial for glucose metabolism.
Furthermore, Maf has been shown to regulate the expression of CD13, a protein found on the surface of endothelial cells. It can activate the CD13 promoter in endothelial cells and facilitate its transcription, while repressing CD13 expression during early stages of myelopoiesis by affecting the interaction between ETS1 and MYB.
During bone development, Maf is involved in the differentiation of chondrocytes and the removal of hypertrophic chondrocytes. It binds to specific promoter sequences in the L7 gene, as well as in the alpha- and beta-crystallin gene promoters, known as Maf response elements (MAREs).
In addition to its role in normal cellular processes, Maf's activity can have different consequences in cancer. Depending on the context, it can act as an oncogene or a tumor suppressor. It binds to antioxidant response element (ARE) sites in the promoters of detoxifying enzyme genes, controlling their expression. When overexpressed, Maf can repress ARE-mediated transcription, potentially disrupting the normal cellular antioxidant defense mechanisms.

Other names

The AS42 oncogene homolog, also known as the Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene, Proto-oncogene c-maf or v maf musculoaponeurotic fibrosarcoma oncogene homolog, is a transcription factor that plays a crucial role in cellular differentiation and survival. Identified as a c-maf proto oncogene in avian musculoaponeurotic fibrosarcoma, MAF is involved in modulating the immune response, regulating cellular proliferation and apoptosis, and controlling differentiation of various tissues and organs.
Also referred to as the v maf musculoaponeurotic fibrosarcoma oncogene homolog in avian species, the MAF2 gene product is highly conserved across species. With MAF_HUMAN being the human homolog of the V-maf musculoaponeurotic fibrosarcoma oncogene, investigations into its function and role in oncogenic transformation have been conducted.
While the cMaf gene is expressed in different tissues, its expression is particularly high in the developing nervous system. Studies suggest that it plays a central role in neuronal differentiation and maintenance of normal brain function. Dysregulation of MAF expression has been linked to several oncogenic processes, including the development of solid tumors such as lung, liver, and breast cancers.
In conclusion, the Proto-oncogene c-maf and its homologues play critical roles in maintaining cellular homeostasis and the development of multiple organ systems. Their dysregulation can disrupt the balance between cellular proliferation and apoptosis, leading to oncogenic transformation and the development of cancer.

Source

Rabbit

Specificity

Maf Antibody detects endogenous levels of Maf

Species Reactivity

Human;Mouse;Rat

Application

WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Immunogen

A synthesized peptide derived from human Maf

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

Target

Background

Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity). When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.

Tissue specificity

Expressed in endothelial cells.

Posttranslational modification

Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by glucocorticoids.Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable). The phosphorylation status can serve to either stimulate or inhibit transcription.

Celluar localization

Nucleus;

UniPort

O75444