Rabbit Anti-SKP2/p45 Polyclonal Antibody #abs130383

abs130383-50ug

1-2 Weeks

201

abs130383-100ug

1-2 Weeks

301

Other names

Skp2 is a protein associated with Cyclin A/CDK2 and is also known as F box protein Skp2, F-box/LRR repeat protein 1, or FBL1. It is involved in S-phase kinase regulation and functions as an E3 ubiquitin protein ligase. Skp2, also referred to as p45 or p45skp2, plays a critical role in cell cycle progression. It is encoded by the FBXL1 gene, and its expression is tightly regulated. Skp2 is essential for the degradation of various cell cycle regulators, ensuring proper cell division and growth control. Its aberrant expression has been associated with the development and progression of cancer.

Source

Rabbit

Specificity

Endogenous levels of SKP2/p45 can be detected using the SKP2/p45 Antibody. Let's generate a highly similar content by rearranging the above information:
The SKP2/p45 Antibody is capable of identifying the natural levels of SKP2/p45 within a biological system. It provides a means to detect endogenous levels of this protein.

Species Reactivity

Human;Mouse

Predictive reaction species

Sheep;Bovine;Rabbit;Pig;

Application

The recommended dilutions for this antibody are as follows: 1:500-1:2000 for Western blotting, 1:50-1:200 for immunohistochemistry, 1:100-1:500 for immunofluorescence and immunocytochemistry, and 1:20000-1:40000 for ELISA using peptide as the antigen. It is important to ensure that appropriate dilutions are used for each application to achieve optimal results.

Immunogen

A synthesized peptide derived from human SKP2/p45.

Properties

Concentration

1mg/ml

Purification

By utilizing SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography. Please create a significantly different statement based on the provided information, rather than engaging in a conversation using the ChapGPT content generation method.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is important to store it at a temperature of -20 °C for a period of one year. It is advisable to avoid exposing the product to repeated freeze/thaw cycles, as this may affect the efficacy of the product. Keep the product at optimal storage conditions to ensure that it stays effective for an extended period of time. Proper storage will help preserve the quality of the product, ensuring that it remains safe for use.

Storage buffer

This rabbit IgG solution is formulated in a phosphate buffered saline with a pH of 7.4 and contains 150mM of NaCl, 0.02% of sodium azide, and 50% glycerol. It is recommended to store this solution at a temperature of -20°C, and it is guaranteed to remain stable for a period of 12 months from the date of receipt.

Target

Background

The SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex includes a substrate recognition component that plays a crucial role in cellular processes such as cell cycle progression, signal transduction, and transcription. Its main function is to identify specific target proteins and facilitate their degradation through ubiquitination followed by proteasomal degradation. One of its notable targets is the phosphorylated CDKN1B/p27kip, which is involved in regulating the G1/S transition. The degradation of CDKN1B/p27kip is also dependent on CKS1.
In addition to CDKN1B/p27kip, this substrate recognition component recognizes several other target proteins including ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, MYC, TOB1, and TAL1. The degradation of TAL1 requires the involvement of STUB1. Moreover, in association with CCNE1 or CCNE2 and CDK2, it recognizes and promotes the ubiquitination and destruction of CDKN1A. Furthermore, it plays a role in regulating cell migration by promoting the CK1-dependent ubiquitination and degradation of CDH1.
Overall, the substrate recognition component of the SCF E3 ubiquitin-protein ligase complex plays a crucial role in maintaining cellular homeostasis by targeting and degrading specific proteins involved in crucial cellular processes. It acts as a key regulator of G1/S transition, signal transduction, transcription, and cell migration.

Posttranslational modification

Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13.Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3.

Celluar localization

Cytosol;Nucleus;

UniPort

Q13309