Rabbit Anti-AGER Polyclonal Antibody #abs125422

abs125422-50ul

In Stock

201

abs125422-100ul

In Stock

301

Other names

The Advanced Glycosylation End Product-Specific Receptor (AGER), also known as RAGE 1 or Renal Tumor Antigen 1, is an important protein involved in recognizing and binding advanced glycosylation end products. AGER is also known as LE 9211 A Antigen, MGC22357, and MOK protein kinase.
Over the years, research has shown that AGER is involved in a wide range of cellular processes, including inflammation, oxidative stress, and cell proliferation. It is also believed to play a role in the development of several chronic diseases, including diabetes, Alzheimer's disease, and cancer.
As a receptor, AGER is responsible for recognizing advanced glycosylation end products, which are formed when sugar molecules bind to proteins or lipids in the body. These end products can accumulate in tissues over time, leading to inflammation and tissue damage.
In addition to its role in recognizing advanced glycosylation end products, AGER also binds to a number of other ligands, including high-mobility group box 1 (HMGB1) and beta-amyloid peptide, both of which are involved in the development of several chronic diseases.
Overall, understanding the role of AGER in cellular processes and disease development is essential for developing new therapies and treatments for a range of chronic disorders.

Source

Rabbit

Specificity

RAGE

Species Reactivity

Human;Mouse;Rat;Dog;Pig;Cow;

Application

The recommended dilutions for WB are between 1:500 and 2000. For ELISA, the recommended dilutions range from 1:500 to 1000. IHC-P dilutions should be between 1:400 and 800, while IHC-F should be diluted to 1:400-800. For ICC, it is recommended to use dilutions between 1:100 and 500. IF requires dilutions of 1:100-500 but note that paraffin sections will require antigen retrieval. These dilution guidelines are based on the original text information.

Immunogen

KLHAGER isoform 1，isoform 241-150/404。，。

Properties

Concentration

1mg/ml

Purification

affinity purified by Protein A

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

To maintain the quality, keep the product stored at a temperature of -20 °C for a year. It is important to avoid subjecting it to multiple freeze/thaw cycles.

Storage buffer

1% BSA, 0.03% Proclin30050%pH7.40.01M TBS。

Research area

Growth Factors and Hormones play a crucial role in Tumor, Cardiovascular, and Cell Biology as well as Neurobiology. These hormonal substances contribute to the development and progression of both benign and malignant tumors. In the field of cardiovascular research, growth factors and hormones have been extensively studied for their involvement in various cardiac diseases such as heart failure and ischemic heart disease. Moreover, understanding the cellular and molecular mechanisms mediated by growth factors and hormones is vital in exploring the pathophysiology of neurobiological disorders like Alzheimer's disease and Parkinson's disease. Additionally, growth factors and hormones have also been linked to diabetes, where they regulate glucose metabolism and insulin production. The study of endocrine diseases further elucidates the role of growth factors and hormones in maintaining homeostasis within the endocrine system. Therefore, a comprehensive understanding of these factors is crucial for advancing our knowledge and developing potential therapeutics in these interconnected fields.

Target

Background

The advanced glycosylation end product-specific receptor (AGER), also known as RAGE, is a cell surface molecule that belongs to the immunoglobulin superfamily. It has the ability to bind molecules that have been modified irreversibly by non-enzymatic glycation and oxidation, which are called advanced glycation end products (AGEs). RAGE is expressed by various cells, including endothelium, mononuclear phagocytes, neurons, and smooth muscle cells.
During development, RAGE is present in high levels, particularly in the central nervous system, but its levels decrease as individuals age. The increased expression of RAGE has been associated with several pathological conditions, such as diabetic vasculopathy, neuropathy, retinopathy, and immune/inflammatory responses in the vessel walls, as well as Alzheimer's disease.
In diabetic tissues, the production of RAGE is influenced by the overproduction of AGEs, which eventually outnumber the protective properties of RAGE. Consequently, it results in oxidative stress and dysfunction of the endothelial cells, leading to vascular diseases in diabetics. Similarly, RAGE also binds to amyloid beta (Ab) in the brain, leading to overstimulation of RAGE. Since Ab is overproduced in the neurons and vessels of Alzheimer's patients' brains, this exacerbates oxidative stress, leading to neuronal degeneration.

Celluar localization

Cell membrane

UniPort

Q15109