Rabbit Anti-M-CSF Receptor Polyclonal Antibody #abs130032

abs130032-50ug

1-2 Weeks

201

abs130032-100ug

1-2 Weeks

301

Description

Granulocyte/macrophage colony-stimulating factors (M-CSF) are cytokines that regulate hematopoiesis by controlling the development, differentiation, and function of two important types of white blood cells – the monocytes-macrophages and the granulocytes. On the other hand, CSF-1 drives the production of cells belonging to the monocyte/macrophage lineage and plays vital roles in various physiological processes such as immune response, bone metabolism, lipoprotein clearance, and reproductive health.
Interestingly, the M-CSF is a homodimer or heterodimer molecule that is linked by disulfide bonds. Moreover, there are three human isoforms of the protein that are produced by a process called alternative splicing. It's important to note that these isoforms are highly similar but possess small differences in their amino acid sequences, which in turn can impact their biological activity.
In summary, the M-CSF and CSF-1 are essential cytokines that regulate the development of various immune cells and have diverse physiological functions in the body. Their proper functioning is critical for maintaining homeostasis and overall health.

Other names

The CD115 antigen, also known as CSF-1 receptor or CFMS, is encoded by the CSF1R gene. It falls under the category of receptor tyrosine kinases with the enzyme classification EC 2.7.10.1. This receptor plays a crucial role in the regulation of macrophage development, survival, and function. It is also involved in the pathogenesis of certain diseases such as hereditary diffuse leukoencephalopathy with spheroids (HDLS). The FMS proto-oncogene, or v-fms, is a viral oncogene homolog that is associated with McDonough feline sarcoma virus. It is worth mentioning that this gene has been previously referred to as FIM2. Furthermore, it is commonly known as M-CSF receptor, M-CSF-R, or MCSFR due to its interaction with macrophage colony-stimulating factor 1 (M-CSF).

Source

Rabbit

Specificity

Detection of total M-CSF Receptor at endogenous levels is possible with the use of the M-CSF Receptor antibody. The specificity of the antibody allows for accurate detection and analysis of the receptor, providing valuable information for research and diagnostic purposes.

Species Reactivity

Human;Mouse;Rat

Application

The suggested dilutions for our antibody are as follows: for western blotting, a dilution of 1:500 to 1:3000 is recommended; for immunohistochemistry, a dilution of 1:50 to 1:200 is appropriate; for immunofluorescence and immunocytochemistry, a dilution of 1:100 to 1:500 is suggested. Finally, for ELISA using the peptide, we recommend a dilution of 1:20000 to 1:40000. Please note that proper optimization may be necessary depending on individual experimental conditions.

Immunogen

M-CSF。，。

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to carry out peptide affinity chromatography for the purification of the antiserum.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of your product, keep it stored at a temperature of -20 °C for a year. It is crucial to avoid exposing the product to repeated freeze/thaw cycles as this can damage its integrity and usefulness. Remember to follow these storage guidelines to get the most out of your product.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.

Tissue specificity

Expressed in bone marrow and in differentiated blood mononuclear cells.

Posttranslational modification

Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-699 and Tyr-923 is important for interaction with GRB2. Phosphorylation at Tyr-723 is important for interaction with PIK3R1. Phosphorylation at Tyr-708 is important for normal receptor degradation. Phosphorylation at Tyr-723 and Tyr-809 is important for interaction with PLCG2. Phosphorylation at Tyr-969 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation.Ubiquitinated. Becomes rapidly polyubiquitinated after autophosphorylation, leading to its degradation.

Celluar localization

Nucleus;Plasma Membrane;

UniPort

P07333