Bax Antibody #abs130033

abs130033-50ug

1-2 Weeks

201

abs130033-100ug

1-2 Weeks

301

Description

BAX is a protein that plays a crucial role in programmed cell death. It achieves this by binding to and counteracting the apoptosis repressor BCL2, or its adenovirus homolog E1B 19k protein. Consequently, it triggers the release of cytochrome c, CASP3 activation, and ultimately apoptosis. BAX belongs to the Bcl-2 family and can form a homodimer or heterodimers with various other family members, including BCL2, E1B 19k protein, BCL2L1 isoform Bcl-X(L), MCL1, and A1. Additionally, it interacts with SH3GLB1, HN, SFN, and YWHAZ, where the latter two cytoplasmic interactions release BAX to mitochondria under stress conditions. Moreover, isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). There are eight different isoforms of the human protein, which arise from alternative splicing.

Other names

BAX is a critical regulator of apoptosis, also known as programmed cell death. It plays a crucial role in maintaining the balance between cell survival and cell death. The BAX protein, encoded by the BAX gene in humans, is a key player in the apoptotic pathway.
The BAX protein is often referred to as Bax-protein or BCL2 associated X protein. Its primary sequence is denoted by the BAX_HUMAN code, and it is alternatively known as BAXA. There are several variants of the BAX protein, such as Baxdelta2G9, Baxdelta2G9omega, and Baxdelta2omega. These variants are derived from the BAX gene and possess specific functional characteristics.
One of the notable variants is BCL2 associated X protein transcript variant delta2, which is abbreviated as Baxdelta2. This particular variant is involved in apoptotic processes and exhibits distinct properties compared to other BAX protein variants. Additionally, Baxdelta2 is also referred to as Bcl-2-like protein 4 or Bcl2-L-4.
The BAX gene gives rise to different isoforms of the BAX protein. Among these isoforms, the membrane isoform alpha is of particular interest. It is responsible for regulating apoptosis at the mitochondrial membrane, thereby influencing the fate of the cell.
In summary, the BAX protein and its variants, including Baxdelta2, play critical roles in apoptosis regulation. The membrane isoform alpha of the BAX protein is especially noteworthy due to its involvement in mitochondrial-mediated cell death.

Source

Rabbit

Specificity

Endogenous levels of total Bax can be accurately detected by the Bax antibody. A similar content can be generated by rearranging the original text as follows: The Bax antibody is capable of specifically detecting the levels of total Bax expressed naturally within the organism.

Species Reactivity

Human;Mouse;Rat

Application

You can use this antibody for multiple applications with different dilutions depending on your experiment requirements. For Western Blotting, the suggested dilution range is 1:500 to 1:3000. For Immunofluorescence and Immunocytochemistry, a recommended dilution range is between 1:100 to 1:500. Additionally, for peptide ELISA, the ideal dilution factor is suggested at 1:20000 to 1:40000. Be sure to optimize the antibody concentration for your specific assay.

Immunogen

A synthesized peptide derived from human Bax.

Properties

Concentration

1mg/ml

Purification

Using SulfoLink™ Coupling Resin, we purified the antiserum through peptide affinity chromatography. Our process resulted in a highly purified solution that is effective in its intended purpose.

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of the product, it is recommended to store it at -20 °C for a period of one year. It is essential to take precautions and avoid subjecting the product to repeated freeze/thaw cycles, as doing so may affect its properties and functionality. It is important to follow the recommended storage conditions to ensure the best possible performance of the product.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline with a pH of 7.4. It also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at a temperature of -20 °C. The stability of the Rabbit IgG is guaranteed for a period of 12 months from the date of receipt.

Target

Background

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Tissue specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Celluar localization

Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Mitochondrion;Nucleus;

UniPort

Q07812