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Rabbit Anti-SREBP2 Polyclonal Antibody#abs137081

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Description

Catalog-specification	Delivery time	USD price
abs137081-100ug	1-2 Weeks	301.0
abs137081-50ug	1-2 Weeks	201.0

Overview
catalog	abs137081
Other names	The Class D basic helix-loop-helix protein 2, also known as SREBP-2 or the Sterol regulatory element binding transcription factor 2, is a crucial protein involved in the regulation of lipid metabolism. Commonly referred to as SREBF 2 or Srebf2, this protein plays a key role in the control of cholesterol and fatty acid biosynthesis. It is composed of a basic helix-loop-helix domain and a DNA-binding domain, making it capable of binding to specific DNA sequences and modulating gene expression. SREBP2 protein contributes to the development of various diseases including obesity, atherosclerosis and diabetes. The processed sterol regulatory element-binding protein 2 is associated with the SREBP-2 regulatory activity and evolutionarily conserved across several species. Sterol regulatory element binding factor 2 is also known as SREBP2gc, a processed isoform of SREBP-2.
Source	Rabbit
Specificity	The total levels of endogenous SREBP2 can be detected using the SREBP2 Antibody. Please rearrange the content according to the original information and generate a highly similar text.
Species Reactivity	Human;Mouse;Rat
Antigen	SREBP2
Application	Here are some rephrased versions of the original text: - The recommended dilution for Western blot is between 1:1000 and 1:3000, while for immunohistochemistry it is 1:200. As for ELISA using a peptide, the recommended dilution ranges from 1:20000 to 1:40000. - To perform Western blotting, it is advised to dilute the sample at a ratio of 1:1000 to 1:3000, whereas for immunohistochemistry a dilution of 1:200 is recommended. ELISA with a peptide as the antigen should be conducted using dilutions ranging from 1:20000 to 1:40000. - If you're planning to do Western blotting, you should use a dilution of 1:1000 to 1:3000, while for immunohistochemistry a dilution of 1:200 is appropriate. In the case of ELISA with a peptide as the antigen, dilutions of 1:20000 to 1:40000 are suggested.
Immunogen	A synthesized peptide derived from human SREBP2.
MW	124kDa
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin was used to purify the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting it to multiple freeze/thaw cycles.
Storage buffer	The provided information states that Rabbit IgG is stored in a solution containing phosphate buffered saline at a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and remains stable for 12 months from the date of receipt.
Target
Background	Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
Tissue specificity	Ubiquitously expressed in adult and fetal tissues.
Posttranslational modification	At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
Celluar localization	Cytosol;Endoplasmic reticulum;Golgi apparatus;Mitochondrion;Nucleus;
UniPort	Q12772