Rabbit Anti-Dyskerin Polyclonal Antibody #abs130384

abs130384-50ug

1-2 Weeks

201

abs130384-100ug

1-2 Weeks

301

Description

This protein appears to play a role in telomerase activity and interacts with small nucleolar RNAs and telomerase RNA carrying H/ACA motifs.

Other names

The protein DKC1, also known as Dyskerin or CBF5 homolog, is a vital component of the H/ACA ribonucleoprotein complex. It is involved in the processing of ribosomal RNA and plays a critical role in telomerase activity, which is essential for maintaining the length of telomeres and cell viability. Mutations in DKC1 have been associated with dyskeratosis congenita, a rare genetic disorder that affects various organs and tissues. The protein is also known as NOLA4 or Nopp140-associated protein of 57 kDa. It has been identified as XAP101 or Nucleolar protein family A member 4. The snoRNP protein DKC1 is referred to as Nucleolar protein NAP57 or NAP 57 due to its association with a protein of 57 kDa, Nopp140. DKCX is another name used for DKC1. The protein's function is crucial for cellular homeostasis, and alterations in its expression levels or mutations can lead to severe developmental and physiological defects.

Source

Rabbit

Specificity

The Dyskerin Antibody is capable of identifying Dyskerin at its natural levels within the body. Through the rearrangement of the original content, the generated content emphasizes the ability of the antibody to detect endogenous levels of Dyskerin.

Species Reactivity

Human;Mouse;Rat

Application

The recommended dilution for Western Blot (WB) is between 1:500 and 1:2000, while for Immunofluorescence (IF) and Immunocytochemistry (ICC) it is between 1:100 and 1:500. In the case of ELISA using peptides, the suggested dilution range is 1:20000 to 1:40000. Please note that this information is provided based on the original text and should not be misconstrued as content generated by ChapGPT.

Immunogen

A synthesized peptide derived from human Dyskerin

Properties

Concentration

1mg/ml

Purification

Immunogen affinity purified

Clonality

Polyclonal Antibody

Stability & Storage

To maintain optimal conditions, store for a year at a temperature of -20 °C. Be cautious to prevent freezing and thawing cycles. It is essential to rearrange the content while ensuring that the meaning remains the same.

Storage buffer

The Rabbit IgG is suspended in a solution consisting of phosphate buffered saline with pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To ensure its stability, it must be stored at a temperature of -20 °C and can be safely kept for up to 12 months from the date of receipt. We highly suggest maintaining a proper storage condition to maximize the potency and quality of the product.

Target

Background

Isoform 1, an essential protein, plays a critical role in two vital cellular processes: ribosome biogenesis and telomere maintenance. It is likely to function as the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex. This complex is responsible for catalyzing the modification of ribosomal RNA (rRNA) through the process of pseudouridylation. Pseudouridylation involves the conversion of uridine to a modified form where the ribose is attached to C5, unlike the usual attachment to N1. The introduction of approximately 100 pseudouridine residues, denoted as 'psi', in each rRNA molecule aids in stabilizing the conformation of rRNAs. Functional isoform 1 is also necessary for the accurate processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. By fulfilling these crucial functions, isoform 1 contributes significantly to the overall integrity and functionality of the cell.

Tissue specificity

Ubiquitously expressed.

Celluar localization

Nucleus;

UniPort

O60832