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Rabbit Anti-ADPGK Polyclonal Antibody #abs130392

Please note that the price provided is only for your reference. For detailed pricing information, please reach out to our seller, Vecent. We recommend contacting Vecent for a more accurate quote. Data Examples Western blot analysis on Jurkat cell lysate using ADPGK Antibody This product is for...

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Description

Catalog-specification	Delivery time	USD price
abs130392-50ug	1-2 Weeks	201
abs130392-100ug	1-2 Weeks	301

Please note that the price provided is only for your reference. For detailed pricing information, please reach out to our seller, Vecent. We recommend contacting Vecent for a more accurate quote.

Overview
Description	The enzyme ADPGK is responsible for catalyzing the phosphorylation of D-glucose into D-glucose 6-phosphate by utilizing ADP as the donor for phosphate. Although GDP and CDP can replace ADP, they are less efficient alternatives. ADPGK belongs to the family of ADP-dependent glucokinases and exists as a monomer. There are a total of 6 isoforms of the human protein created through alternative splicing. To summarize, ADPGK is a crucial enzyme in glucose metabolism, and its various isoforms provide additional complexity to its biological function.
Other names	ADP-dependent glucokinase, also known as ADP GK or ADPGK, is encoded by the gene 2610017G09Rik and is an enzyme that plays a crucial role in glucose metabolism. This enzyme is responsible for converting glucose into glucose-6-phosphate in the presence of ADP. ADPGK is also known by other names, including ATP-dependent glucokinase and RbBP-35. The function of ADPGK is essential for maintaining healthy blood sugar levels in the body. As glucose is taken up by the cells, it is converted into glucose-6-phosphate by ADPGK, which can then be further metabolized to produce energy. The regulation of this process is crucial in preventing hyperglycemia and hypoglycemia. ADPGK has also been implicated in the development of some types of cancer, as some tumor cells have been found to overexpress this enzyme. However, more research is needed to fully understand the role of ADPGK in cancer biology. In summary, ADP-dependent glucokinase is an important enzyme in glucose metabolism and cellular energy production. Its proper function is crucial for maintaining healthy blood sugar levels and preventing metabolic disorders.
Source	Rabbit
Specificity	ADPGK Antibody detects endogenous levels of ADPGK.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Horse;Dog;
Application	You can dilute the antibody for Western blotting in a range of 1:500 to 1:2000. For immunofluorescence/immunocytochemistry, the dilution range is suggested to be 1:100 to 1:500. Meanwhile, for ELISA assay using peptide as a coating antigen, the recommended dilution range is 1:20000 to 1:40000. These dilutions can help you achieve optimal results in your experiments.
Immunogen	A synthesized peptide derived from human ADPGK.
Properties
Concentration	1mg/ml
Purification	The SulfoLink™ Coupling Resin was employed for peptide affinity chromatography purification of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to keep it stored at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this could negatively impact its effectiveness and shelf life. So, make sure to handle the product with care and store it appropriately to ensure its optimal performance and longevity.
Storage buffer	This product is Rabbit IgG in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl and 0.02% sodium azide, as well as 50% glycerol. It is recommended to store this product at -20°C. It has a shelf life of 12 months from the date of receipt.
Target
Background	ADP is the phosphate donor in the process of phosphorylating D-glucose to D-glucose 6-phosphate. However, it is worth noting that GDP and CDP can also serve as phosphate donors, although their efficiency is lower compared to ADP. This similarity in function implies that these molecules have comparable roles in catalyzing the reaction.
Celluar localization	Extracellular region or secreted; Endoplasmic reticulum. It is essential to rearrange the given content accurately, as follows: The endoplasmic reticulum is responsible for various cellular functions, including the synthesis and folding of proteins. Once proteins are correctly folded, they can be transported to their destination within the cell or to the extracellular region for secretion. This process ensures that proteins are properly delivered to their designated locations, both inside and outside the cell.
UniPort	Q9BRR6