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Rabbit Anti-MEKKK4 Polyclonal Antibody #abs130389

Please note that the listed price is for your reference only. For detailed pricing information, we kindly advise you to contact our seller, Vecent. Data Examples Western blot analysis on HuvEc cell lysate using MEKKK 4 Antibody This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs130389-50ug	1-2 Weeks	201
abs130389-100ug	1-2 Weeks	301

Please note that the listed price is for your reference only. For detailed pricing information, we kindly advise you to contact our seller, Vecent.

Overview
Description	This protein belongs to the STE20 subfamily of Ser/Thr kinases and may have a crucial role in responding to environmental stress and cytokines like TNF-alpha. It is known to interact with adapter proteins Nck's SH3 domain and binds to the N-terminal region of SPG3A via its CNH regulatory domain. Up-regulated in primary tumors and cell lines, it is required for anchorage-independent growth and ras-dependent focus formation in multiple cell types. There exist 5 isoforms of this protein generated by alternative splicing.
Other names	FLH21957; FLJ10410; FLJ20373; FLJ90111; Germline center-like kinase kinase; HGK; HPK/GCK like kinase; HPK/GCK like kinase HGK; HPK/GCK-like kinase HGK; KIAA0687; M4K4_HUMAN; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Mitogen-activated protein kinase kinase kinase kinase 4; Kinase interacting with Nck; Nck-interacting kinase; NIK. Please rearrange the above information to generate a highly similar content, based on the original text: The following is a list of proteins: FLH21957, FLJ10410, FLJ20373, FLJ90111, Germline center-like kinase kinase, HGK, HPK/GCK-like kinase, HPK/GCK-like kinase HGK, HPK/GCK-like kinase HGK, KIAA0687, M4K4_HUMAN, MAP4K4, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, MEKKK4, Mitogen-activated protein kinase kinase kinase kinase 4, Mitogen-activated protein kinase kinase kinase kinase 4, Nck-interacting kinase, Nck-interacting kinase, NIK.
Source	Rabbit
Specificity	The endogenous levels of MEKKK 4 can be detected using the MEKKK 4 Antibody. We have developed a highly specific antibody that can accurately detect the presence of MEKKK 4 in biological samples. This antibody is a valuable tool for researchers studying MEKKK 4 and its involvement in various cellular processes. By utilizing this antibody, scientists can gain insights into the expression and function of MEKKK 4 in different tissues and conditions. The MEKKK 4 Antibody provides a reliable and sensitive method for studying the role of MEKKK 4 in biological systems.
Species Reactivity	Human;Mouse
Application	For WB assay, a working dilution of 1:500-1:2000 is recommended. When performing IF or ICC, the suggested dilution range is 1:100-1:500. ELISA using peptides as the target antigen should be performed at a dilution of 1:20000-1:40000. To summarize, these are the recommended dilutions for each type of assay: 1:500-1:2000 for WB, 1:100-1:500 for IF/ICC, and 1:20000-1:40000 for ELISA using peptides.
Immunogen	A synthesized peptide derived from human MEKKK 4
Properties
Concentration	1mg/ml
Purification	Immunogen affinity purified
Clonality	Polyclonal Antibody
Stability & Storage	For a period of one year, this item should be stored at a temperature of -20 °C. In order to prevent damage, it is recommended to avoid exposing it to multiple freeze/thaw cycles. It is important to note that this information is based on the original text and has been rearranged to create a highly similar content.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, with 150mM NaCl, and 0.02% sodium azide. It also contains 50% glycerol and should be stored at -20°C. The product is stable for a period of 12 months from receipt. Please ensure that the conditions for storage are consistent with the information provided.
Target
Background	This kinase, which belongs to the serine/threonine family, may be involved in responding to external stress and cytokines such as TNF-alpha. It is believed to acts as an initiator of the JUN N-terminal pathway. Studies have shown that it is capable of phosphorylating SMAD1 at Thr-322, indicating its potential role in regulating cellular signals.
Tissue specificity	Isoform 4 is predominantly found in the liver, skeletal muscle, and placenta, whereas isoform 5 is widely expressed, with abundance in the brain.
UniPort	O95819